Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4114481	M	M	NA	Agree	0.03	6.12	0.00	38.52	17.43	62.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866112	0.078847	0.036496	0.018545	82.80	5.21	11.56	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114482	M	M	NA	Agree	0.02	4.16	0.00	30.45	11.78	61.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883586	0.046236	0.035449	0.034729	81.41	8.37	9.69	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114483	M	M	NA	Agree	0.03	3.66	0.00	31.54	11.59	38.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903	0.05984	0.028443	0.008717	89.30	4.10	6.15	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114484	M	M	NA	Agree	0.00	3.18	0.00	28.77	7.49	45.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.926738	0.038938	0.027313	0.007012	83.49	4.43	11.61	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114485	M	M	NA	Agree	0.06	6.24	0.00	38.03	16.54	66.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856484	0.075763	0.036272	0.031481	89.36	6.89	3.29	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114486	M	M	NA	Agree	0.01	6.01	0.00	47.66	13.62	69.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886785	0.061773	0.033041	0.018401	90.37	5.57	3.62	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114487	M	M	NA	Agree	0.04	8.27	0.00	48.34	18.64	82.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862915	0.08274	0.038328	0.016016	90.92	5.42	3.18	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114488	M	M	NA	Agree	0.01	6.51	0.00	40.92	12.38	58.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.901766	0.057525	0.032023	0.008686	91.18	4.76	3.60	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114489	M	M	NA	Agree	0.04	9.20	0.00	42.53	21.89	66.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848844	0.100386	0.043785	0.006985	85.88	8.58	4.69	243	LP2 (2026-09-30)	PASS	NonPolyA_perM 243 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114490	M	M	NA	Agree	0.03	5.89	0.00	31.60	18.35	59.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868481	0.080951	0.04202	0.008547	87.97	4.60	7.02	161	LP2 (2026-09-30)	PASS	NonPolyA_perM 161 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114491	M	M	NA	Agree	0.06	6.58	0.00	36.15	21.60	58.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860628	0.089724	0.041909	0.007738	87.07	4.50	8.02	193	LP2 (2026-09-30)	PASS	NonPolyA_perM 193 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114492	M	M	NA	Agree	0.05	6.24	0.00	29.35	19.67	68.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87433	0.075551	0.042648	0.00747	85.52	5.17	8.86	163	LP2 (2026-09-30)	PASS	NonPolyA_perM 163 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114493	M	M	NA	Agree	0.03	8.09	0.00	33.77	19.40	62.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856646	0.092483	0.040709	0.010163	85.30	9.36	4.46	198	LP2 (2026-09-30)	PASS	NonPolyA_perM 198 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114494	M	M	NA	Agree	0.03	6.56	0.00	34.27	19.47	52.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865882	0.092386	0.037077	0.004654	79.80	7.63	11.87	173	LP2 (2026-09-30)	PASS	NonPolyA_perM 173 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114495	M	M	NA	Agree	0.06	10.24	0.00	45.23	25.40	73.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834314	0.110968	0.044738	0.00998	87.94	7.28	4.09	240	LP2 (2026-09-30)	PASS	NonPolyA_perM 240 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4114496	M	M	NA	Agree	0.09	10.90	0.00	39.14	20.51	74.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846271	0.100976	0.045637	0.007115	86.93	8.61	3.50	271	LP2 (2026-09-30)	PASS	NonPolyA_perM 271 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does