Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4083616	M	M	NA	Agree	0.00	2.23	0.00	17.47	4.57	60.50	NONE	POLYA	0.735901	0.025168	0.0291	0.209831	75.99	20.83	3.04	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4083617	M	M	NA	Agree	0.00	1.91	0.00	14.54	2.80	62.44	NONE	POLYA	0.799675	0.024871	0.029189	0.146264	82.13	15.04	2.69	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4083618	M	M	NA	Agree	0.00	1.69	0.00	13.31	2.85	58.49	NONE	POLYA	0.788665	0.023282	0.028285	0.159768	80.43	16.30	3.14	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4083619	M	M	NA	Agree	0.00	2.82	0.00	17.92	3.61	65.03	NONE	POLYA	0.788281	0.025257	0.027476	0.158986	81.33	15.76	2.77	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4083620	M	F	NA	Conflict	11.67	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.8227	0.027066	0.028307	0.121927	85.14	12.51	2.22	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4083621	M	M	NA	Agree	0.00	2.72	0.00	20.12	4.70	56.70	NONE	POLYA	0.773014	0.027025	0.029213	0.170748	80.20	17.14	2.50	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does