Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4026196	M	M	NA	Agree	0.12	3.57	0.00	27.26	4.83	34.23	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.835534	0.065274	0.024398	0.074793	61.42	7.99	30.20	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026197	M	M	NA	Agree	0.04	2.69	0.00	29.48	7.75	37.86	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.867994	0.064715	0.025019	0.042272	65.62	5.39	28.65	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026198	M	M	NA	Agree	0.09	2.17	0.00	18.30	5.00	26.46	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.86706	0.051084	0.021745	0.060111	64.98	6.77	27.88	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026199	M	M	NA	Agree	0.09	4.01	0.00	23.89	5.83	29.24	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.821989	0.065583	0.023295	0.089133	63.85	9.29	26.45	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026200	M	M	NA	Agree	0.11	4.17	0.00	25.64	6.21	36.26	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.785893	0.069965	0.023913	0.12023	63.90	12.39	23.40	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026201	M	M	NA	Agree	0.05	2.84	0.00	29.57	5.33	30.30	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.836001	0.069957	0.025124	0.068917	63.13	7.47	28.99	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026202	M	M	NA	Agree	0.08	2.43	0.00	23.26	6.76	22.38	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.888099	0.063218	0.025447	0.023235	65.73	3.54	30.37	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026203	M	M	NA	Agree	0.10	2.90	0.00	30.00	5.07	34.07	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.890189	0.060388	0.025555	0.023868	67.22	3.99	28.40	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026204	M	M	NA	Agree	0.10	3.72	0.00	31.88	7.06	31.60	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.896438	0.059137	0.024939	0.019485	63.73	3.35	32.53	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026205	M	M	NA	Agree	0.05	3.44	0.00	28.46	4.81	31.62	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.866113	0.057844	0.023375	0.052668	65.38	6.38	27.81	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026206	M	M	NA	Agree	0.11	3.49	0.00	29.84	6.24	33.88	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.848444	0.050763	0.022675	0.078118	63.50	8.38	27.73	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026207	M	M	NA	Agree	0.11	5.31	0.00	33.39	4.71	41.11	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.814911	0.065383	0.023307	0.096399	62.89	10.20	26.49	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026208	M	M	NA	Agree	0.12	3.96	0.00	28.93	6.80	31.62	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.872987	0.070977	0.025334	0.030702	67.85	4.38	27.37	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026209	M	M	NA	Agree	0.15	4.19	0.00	27.32	5.84	36.67	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.859897	0.053496	0.023526	0.063081	64.80	7.32	27.46	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4026210	M	M	NA	Agree	0.09	3.57	0.00	34.10	5.83	32.91	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.882051	0.058409	0.024176	0.035365	66.99	5.03	27.58	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does