Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM3613406	M	M	NA	Agree	0.06	1.60	0.00	16.26	4.87	34.70	NONE	POLYA	0.883095	0.079942	0.033448	0.003516	93.15	2.70	3.74	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613407	M	M	NA	Agree	0.06	1.57	0.00	13.51	4.04	29.89	NONE	POLYA	0.885078	0.077607	0.03136	0.005955	93.26	2.82	3.56	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613408	M	M	NA	Agree	0.02	2.06	0.00	13.86	4.19	30.48	NONE	POLYA	0.92847	0.04209	0.026204	0.003236	94.70	2.57	2.35	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613409	M	M	NA	Agree	0.21	2.19	0.00	16.15	5.99	38.45	NONE	POLYA	0.873017	0.086844	0.035262	0.004877	92.64	2.59	4.45	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613410	M	M	NA	Agree	0.06	1.64	0.00	16.42	5.16	31.96	NONE	POLYA	0.873198	0.092295	0.030062	0.004445	93.58	2.57	3.39	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613411	M	M	NA	Agree	0.07	1.98	0.00	16.28	6.24	32.52	NONE	POLYA	0.885825	0.081487	0.030433	0.002255	93.96	2.43	3.08	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613412	M	M	NA	Agree	0.09	1.75	0.00	14.46	4.96	30.29	NONE	POLYA	0.896638	0.070811	0.029669	0.002882	93.71	2.40	3.28	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613413	M	M	NA	Agree	0.09	1.96	0.00	14.27	5.61	34.48	NONE	POLYA	0.879454	0.085084	0.032145	0.003317	93.94	2.50	3.16	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613414	M	M	NA	Agree	0.08	1.45	0.00	15.76	5.18	32.34	NONE	POLYA	0.886343	0.077544	0.031806	0.004308	93.53	2.73	3.20	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613415	M	M	NA	Agree	0.04	1.23	0.00	12.85	3.74	28.30	NONE	POLYA	0.914631	0.052751	0.028125	0.004494	93.55	2.92	3.07	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613416	M	M	NA	Agree	0.11	0.58	0.00	7.82	1.39	27.03	NONE	POLYA	0.918405	0.045898	0.030891	0.004805	93.07	2.81	3.74	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3613417	M	M	NA	Agree	0.03	2.11	0.00	16.66	3.36	34.82	NONE	POLYA	0.928052	0.041144	0.026401	0.004403	94.03	2.60	3.06	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does