Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM3517578	M	M	NA	Agree	0.02	9.28	0.00	51.52	9.02	70.03	NONE	POLYA	0.922923	0.057035	0.019696	0.000346	94.73	1.39	3.77	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3517579	M	M	NA	Agree	0.03	9.53	0.00	47.46	10.53	65.19	NONE	POLYA	0.91564	0.063878	0.02013	0.000352	96.15	1.42	2.32	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3517580	M	M	NA	Agree	0.03	9.74	0.00	47.85	9.49	68.89	NONE	POLYA	0.924332	0.05579	0.019361	0.000517	95.86	1.40	2.63	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3517581	M	M	NA	Agree	0.01	8.62	0.00	45.09	10.04	63.89	NONE	POLYA	0.923147	0.057148	0.019074	0.000631	96.08	1.36	2.44	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3517582	M	M	NA	Agree	0.00	5.83	0.00	33.61	4.86	51.13	NONE	POLYA	0.956438	0.027782	0.015134	0.000645	92.62	1.29	5.99	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3517583	M	M	NA	Agree	0.00	7.58	0.00	40.71	5.52	64.33	NONE	POLYA	0.957391	0.027471	0.014337	0.000801	96.47	1.32	2.10	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3517584	M	M	NA	Agree	0.00	7.15	0.00	40.26	7.10	59.29	NONE	POLYA	0.945021	0.037667	0.016676	0.000636	96.20	1.25	2.46	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3517585	M	M	NA	Agree	0.00	5.48	0.00	34.91	4.99	55.99	NONE	POLYA	0.951081	0.033775	0.01477	0.000375	96.12	1.10	2.69	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does