Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM3333211	F	F	NA	Agree	284.45	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869362	0.085889	0.043446	0.001303	93.99	3.78	1.90	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333212	F	F	NA	Agree	389.62	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844986	0.100607	0.05279	0.001618	93.04	3.57	3.09	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333213	F	F	NA	Agree	246.16	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866267	0.089382	0.043301	0.001049	92.60	5.13	1.93	170	LP2 (2026-09-30)	PASS	NonPolyA_perM 170 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333214	F	F	NA	Agree	231.89	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893179	0.065234	0.040432	0.001155	93.30	4.55	1.85	139	LP2 (2026-09-30)	PASS	NonPolyA_perM 139 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333215	F	F	NA	Agree	265.91	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873089	0.082103	0.042847	0.001961	93.69	4.24	1.78	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333216	F	F	NA	Agree	235.88	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89079	0.061959	0.045772	0.001479	90.60	5.14	3.93	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333217	F	F	NA	Agree	233.61	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872262	0.086456	0.040098	0.001185	92.31	5.43	1.91	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333218	F	F	NA	Agree	235.42	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874066	0.085516	0.03915	0.001268	91.86	5.36	2.44	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333219	F	F	NA	Agree	242.09	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884052	0.07302	0.042134	0.000794	93.44	4.29	1.99	163	LP2 (2026-09-30)	PASS	NonPolyA_perM 163 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3333220	F	F	NA	Agree	261.22	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895087	0.060089	0.043077	0.001747	92.35	4.76	2.64	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does