Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM3181612	M	M	NA	Agree	0.02	1.21	0.00	14.28	5.08	34.32	NONE	POLYA	0.920988	0.043368	0.025134	0.01051	92.11	4.54	2.96	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181613	M	M	NA	Agree	0.00	0.68	0.00	13.35	5.14	27.13	NONE	POLYA	0.910561	0.049841	0.029434	0.010165	93.15	4.42	2.00	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181614	M	M	NA	Agree	0.02	0.96	0.00	18.33	4.49	33.20	NONE	POLYA	0.889996	0.055848	0.030513	0.023643	92.10	5.65	1.79	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181615	M	M	NA	Agree	0.00	1.04	0.00	11.51	4.78	31.49	NONE	POLYA	0.926184	0.041302	0.022661	0.009853	94.07	3.80	1.78	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181616	M	M	NA	Agree	0.01	1.03	0.00	10.10	4.34	24.92	NONE	POLYA	0.868564	0.0611	0.024878	0.045458	90.36	7.31	1.95	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181617	M	F	NA	Conflict	44.25	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.891678	0.051508	0.025082	0.031732	90.51	6.88	2.24	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181618	M	M	NA	Agree	0.00	0.50	0.00	9.12	2.94	18.55	NONE	POLYA	0.697458	0.04626	0.023135	0.233147	71.29	25.52	2.72	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181619	M	M	NA	Agree	0.00	1.33	0.00	13.63	5.81	34.93	NONE	POLYA	0.913477	0.051811	0.027514	0.007198	92.60	4.31	2.60	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181620	M	M	NA	Agree	0.00	0.61	0.00	6.48	2.73	14.36	NONE	POLYA	0.813638	0.045812	0.023643	0.116907	82.72	14.54	2.35	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181621	M	M	NA	Agree	0.00	1.07	0.00	10.12	4.85	26.06	NONE	POLYA	0.918546	0.044496	0.025761	0.011196	92.20	4.62	2.71	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181622	M	M	NA	Agree	0.01	0.86	0.00	9.12	3.27	25.56	NONE	POLYA	0.846968	0.047306	0.024199	0.081527	86.16	10.90	2.50	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181623	F	M	NA	Conflict	0.01	0.33	0.00	3.44	1.44	8.79	NONE	POLYA	0.529053	0.040253	0.014811	0.415884	54.97	42.82	1.80	201	LP2 (2026-09-30)	PASS	NonPolyA_perM 201 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181624	F	M	NA	Conflict	0.00	1.18	0.00	10.31	5.97	33.99	NONE	POLYA	0.914858	0.044907	0.023219	0.017016	93.01	4.43	2.20	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181625	F	M	NA	Conflict	0.00	0.79	0.00	11.53	5.28	22.45	NONE	POLYA	0.918507	0.044174	0.023964	0.013356	93.64	3.97	2.06	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181626	F	M	NA	Conflict	0.00	1.33	0.00	12.31	5.49	27.86	NONE	POLYA	0.928309	0.03982	0.022228	0.009643	93.61	4.03	2.03	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181627	F	F	NA	Agree	34.59	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.852226	0.048301	0.021145	0.078328	86.25	11.09	2.37	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181628	F	F	NA	Agree	39.86	0.00	0.00	0.04	0.00	0.00	NONE	POLYA	0.916446	0.04515	0.024439	0.013965	91.84	5.49	2.33	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181629	F	F	NA	Agree	40.94	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.866018	0.049985	0.023674	0.060324	88.24	9.45	1.95	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181630	F	F	NA	Agree	35.49	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.930468	0.038996	0.022362	0.008174	93.55	4.24	1.93	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181631	F	F	NA	Agree	32.20	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.928906	0.038442	0.02263	0.010022	92.90	4.50	2.30	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181632	F	F	NA	Agree	60.09	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.886524	0.053905	0.028857	0.030714	91.14	6.67	1.95	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3181633	F	F	NA	Agree	53.78	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.891792	0.053725	0.027245	0.027238	91.57	6.17	1.98	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does