Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2994929	M	M	NA	Agree	0.01	4.27	0.00	25.34	5.38	46.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90366	0.023722	0.022202	0.050416	89.09	7.86	2.70	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994930	M	M	NA	Agree	0.00	2.82	0.00	21.98	3.31	35.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921402	0.017837	0.018761	0.042	90.87	6.73	2.05	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994931	M	M	NA	Agree	0.00	2.95	0.00	22.31	3.53	35.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925032	0.017496	0.01768	0.039792	90.99	6.33	2.38	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994932	M	M	NA	Agree	0.00	2.90	0.00	21.94	3.96	34.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915841	0.02094	0.019258	0.043961	90.51	6.84	2.33	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994933	M	M	NA	Agree	0.00	2.81	0.00	21.54	4.27	37.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920878	0.019226	0.019156	0.040741	90.16	6.64	2.90	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994934	M	M	NA	Agree	0.01	2.76	0.00	21.86	4.77	41.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909962	0.021374	0.020161	0.048502	91.19	7.40	1.16	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994935	M	M	NA	Agree	0.00	2.57	0.00	21.26	3.68	34.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916238	0.02021	0.019114	0.044437	90.42	6.87	2.43	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994936	M	M	NA	Agree	0.00	2.70	0.00	21.76	4.90	37.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923753	0.022564	0.020949	0.032733	92.09	5.88	1.79	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994937	M	M	NA	Agree	0.00	3.89	0.00	29.11	5.34	49.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911439	0.021197	0.021416	0.045949	89.17	7.19	3.34	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994938	M	M	NA	Agree	0.00	3.80	0.00	24.36	5.09	43.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915481	0.022833	0.021634	0.040052	90.93	6.62	2.14	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994939	M	M	NA	Agree	0.00	2.82	0.00	22.73	3.74	41.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911046	0.016121	0.018691	0.054141	88.48	7.77	3.45	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994940	M	M	NA	Agree	0.00	3.65	0.00	22.73	4.61	44.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915038	0.021335	0.020392	0.043235	90.69	6.98	2.02	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994941	M	M	NA	Agree	0.00	3.80	0.00	25.43	3.56	44.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915847	0.017348	0.018522	0.048283	90.27	7.35	2.11	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994942	M	M	NA	Agree	0.00	3.76	0.00	23.84	4.41	42.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911907	0.02186	0.01973	0.046503	89.89	7.15	2.63	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994943	M	M	NA	Agree	0.00	4.03	0.00	26.45	4.88	41.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916689	0.022722	0.02075	0.03984	91.10	6.72	1.90	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994944	M	M	NA	Agree	0.00	3.01	0.00	23.97	3.77	42.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913645	0.018786	0.0193	0.048269	88.53	7.19	3.99	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994945	M	M	NA	Agree	0.00	2.54	0.00	20.71	3.94	37.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91574	0.018778	0.019182	0.0463	90.58	7.16	1.98	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994946	M	M	NA	Agree	0.01	4.34	0.00	26.24	6.50	51.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.910092	0.026076	0.022514	0.041318	89.95	7.09	2.64	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994947	M	M	NA	Agree	0.00	3.65	0.00	22.89	5.02	40.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924633	0.019542	0.020292	0.035533	91.61	6.45	1.68	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994948	M	M	NA	Agree	0.00	3.28	0.00	23.26	3.86	45.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913955	0.019002	0.020013	0.04703	90.14	7.32	2.27	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994949	M	M	NA	Agree	0.00	2.28	0.00	20.73	4.65	40.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925087	0.01878	0.018609	0.037523	90.86	6.39	2.48	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994950	M	M	NA	Agree	0.01	4.53	0.00	25.09	8.23	48.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899018	0.030901	0.023942	0.046139	88.95	7.51	3.22	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994951	M	M	NA	Agree	0.00	3.07	0.00	25.59	4.14	38.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913751	0.020905	0.019287	0.046057	90.65	7.20	1.85	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994952	M	M	NA	Agree	0.01	3.41	0.00	13.31	4.88	34.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918606	0.020481	0.022457	0.038457	89.96	6.93	2.78	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994953	M	M	NA	Agree	0.01	3.51	0.00	26.21	4.88	45.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916565	0.018937	0.020402	0.044095	90.16	7.21	2.32	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994954	M	M	NA	Agree	0.00	3.64	0.00	21.02	5.42	38.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915524	0.026356	0.022677	0.035442	90.24	6.47	2.99	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994955	M	M	NA	Agree	0.00	3.07	0.00	21.48	3.53	35.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924404	0.01743	0.018003	0.040163	91.06	6.60	2.06	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2994956	M	M	NA	Agree	0.00	3.21	0.00	28.26	4.22	43.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.922928	0.021246	0.020361	0.035466	91.34	6.30	2.06	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does