Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2982096	M	M	NA	Agree	0.00	2.40	0.00	22.71	4.89	94.63	NONE	POLYA	0.874037	0.037371	0.065886	0.022707	85.83	8.33	5.02	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2982097	M	M	NA	Agree	0.00	3.04	0.00	24.74	4.12	95.58	NONE	POLYA	0.831464	0.054285	0.087546	0.026706	85.37	8.36	5.37	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2982098	M	M	NA	Agree	0.00	2.41	0.00	23.84	4.68	87.28	NONE	POLYA	0.860466	0.040358	0.071074	0.028101	86.46	8.47	4.39	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2982099	M	M	NA	Agree	0.00	2.76	0.00	22.11	3.73	90.04	NONE	POLYA	0.824201	0.054377	0.087948	0.033475	85.43	8.87	4.85	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2982100	M	M	NA	Agree	0.04	1.72	0.00	15.31	3.17	69.86	NONE	POLYA	0.766703	0.078485	0.117687	0.037126	84.01	9.14	6.04	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2982101	M	M	NA	Agree	0.00	1.72	0.00	14.27	2.69	80.51	NONE	POLYA	0.859901	0.036601	0.072368	0.03113	85.19	9.33	4.78	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2982102	M	M	NA	Agree	0.00	2.25	0.00	23.07	5.19	81.37	NONE	POLYA	0.769377	0.088518	0.117892	0.024213	85.79	8.02	5.17	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2982103	M	M	NA	Agree	0.00	1.70	0.00	23.83	4.07	91.41	NONE	POLYA	0.809383	0.064847	0.093069	0.032701	84.62	9.26	5.27	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does