Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2943055	M	M	NA	Agree	0.01	9.04	0.00	41.60	27.74	75.76	NONE	POLYA	0.881101	0.072441	0.046421	0.000037	94.34	1.16	4.32	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943056	M	M	NA	Agree	0.01	10.15	0.00	52.98	30.71	96.65	NONE	POLYA	0.89109	0.062814	0.046047	0.000049	94.21	1.13	4.49	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943057	M	M	NA	Agree	0.00	7.78	0.00	41.44	24.49	73.53	NONE	POLYA	0.888642	0.062355	0.046212	0.00279	92.38	1.60	5.85	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943058	M	M	NA	Agree	0.00	8.96	0.00	45.73	26.01	71.93	NONE	POLYA	0.879038	0.073088	0.047849	0.000025	95.40	1.15	3.27	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943059	M	M	NA	Agree	0.00	8.54	0.00	41.76	28.39	72.43	NONE	POLYA	0.879225	0.07202	0.048734	0.000021	96.19	1.19	2.44	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943060	M	M	NA	Agree	0.00	8.80	0.00	42.17	26.90	68.96	NONE	POLYA	0.880269	0.071472	0.048233	0.000026	96.15	1.19	2.48	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943061	M	M	NA	Agree	0.01	8.67	0.00	38.29	25.98	77.35	NONE	POLYA	0.886189	0.066734	0.047062	0.000015	94.22	1.17	4.43	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943062	M	M	NA	Agree	0.00	8.47	0.00	39.30	25.85	71.18	NONE	POLYA	0.889272	0.062513	0.048195	0.00002	96.24	1.20	2.39	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943063	M	M	NA	Agree	0.00	8.78	0.00	44.65	24.81	70.29	NONE	POLYA	0.882636	0.06898	0.048181	0.000203	96.00	1.25	2.55	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943064	M	M	NA	Agree	0.00	9.29	0.00	44.55	26.16	72.71	NONE	POLYA	0.868981	0.081835	0.049077	0.000107	94.56	1.19	4.06	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943065	M	M	NA	Agree	0.00	9.54	0.00	43.26	27.29	69.75	NONE	POLYA	0.877568	0.075279	0.047045	0.000107	94.52	1.15	4.14	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2943066	M	M	NA	Agree	0.00	9.62	0.00	48.21	25.18	79.87	NONE	POLYA	0.875965	0.07697	0.046989	0.000077	94.56	1.14	4.14	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does