Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2532864	M	M	NA	Agree	0.00	6.53	0.00	48.77	18.87	94.93	NONE	POLYA	0.87648	0.040321	0.039943	0.043257	76.25	5.07	18.55	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532865	M	M	NA	Agree	0.00	7.51	0.00	50.11	17.89	94.87	NONE	POLYA	0.880848	0.036264	0.039393	0.043495	78.35	5.15	16.37	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532866	M	M	NA	Agree	0.00	5.96	0.00	45.72	15.98	85.91	NONE	POLYA	0.869716	0.046989	0.040124	0.04317	78.56	5.19	16.10	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532867	M	M	NA	Agree	0.00	8.34	0.00	50.19	17.23	110.80	NONE	POLYA	0.874835	0.039641	0.041778	0.043746	78.25	5.19	16.41	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532868	M	M	NA	Agree	0.00	7.62	0.00	55.04	20.67	92.17	NONE	POLYA	0.879289	0.039636	0.040167	0.040907	78.63	4.83	16.40	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532869	M	M	NA	Agree	0.00	5.77	0.00	58.00	20.12	94.30	NONE	POLYA	0.87964	0.037495	0.040146	0.042719	78.83	5.00	16.02	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532870	M	M	NA	Agree	0.00	6.39	0.00	59.56	17.77	96.74	NONE	POLYA	0.87096	0.042291	0.04156	0.045189	77.08	5.19	17.59	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532871	M	M	NA	Agree	0.00	7.71	0.00	65.57	17.45	95.06	NONE	POLYA	0.877897	0.039143	0.040969	0.041991	77.26	4.93	17.64	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532872	M	M	NA	Agree	0.00	7.75	0.00	54.07	22.30	92.96	NONE	POLYA	0.873466	0.040599	0.040701	0.045234	78.56	5.16	16.15	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532873	M	M	NA	Agree	0.00	6.36	0.00	47.87	21.57	93.66	NONE	POLYA	0.87126	0.044414	0.040467	0.043859	77.10	4.99	17.77	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532874	M	M	NA	Agree	0.00	5.24	0.00	56.60	19.95	88.27	NONE	POLYA	0.875537	0.039374	0.039282	0.045808	77.45	5.23	17.17	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2532875	M	M	NA	Agree	0.00	6.60	0.00	57.03	18.97	95.33	NONE	POLYA	0.873724	0.040006	0.039642	0.046629	77.87	5.35	16.64	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does