Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2514229	M	M	NA	Agree	0.00	5.69	0.00	34.22	14.29	63.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85989	0.069139	0.051713	0.019258	93.11	4.84	1.68	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514230	M	M	NA	Agree	0.00	5.59	0.00	32.02	12.59	61.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855406	0.072325	0.051825	0.020444	92.57	4.95	2.09	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514231	M	M	NA	Agree	0.03	4.38	0.00	30.53	12.91	61.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844717	0.07519	0.051989	0.028104	92.06	5.64	1.92	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514232	M	M	NA	Agree	0.01	4.68	0.00	27.44	11.49	51.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.840593	0.081266	0.051632	0.026509	92.15	5.53	1.95	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514233	M	M	NA	Agree	0.01	3.92	0.00	28.10	10.80	56.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8614	0.053898	0.05148	0.033221	91.52	6.18	1.94	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514234	M	M	NA	Agree	0.01	4.13	0.00	28.02	11.48	54.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869662	0.052874	0.051589	0.025875	91.25	5.37	3.05	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514235	M	M	NA	Agree	0.00	3.47	0.00	25.33	8.87	46.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860721	0.050598	0.052817	0.035864	90.72	6.45	2.47	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514236	M	M	NA	Agree	0.00	3.82	0.00	29.95	10.78	52.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8595	0.053542	0.052251	0.034707	90.46	6.28	2.92	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514237	M	M	NA	Agree	0.02	3.06	0.00	26.02	8.82	48.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866709	0.047245	0.052797	0.033249	90.84	6.22	2.59	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514238	M	M	NA	Agree	0.01	4.05	0.00	28.32	10.61	55.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855797	0.063132	0.051643	0.029428	91.63	5.82	2.20	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514239	M	M	NA	Agree	0.01	3.46	0.00	27.21	10.78	55.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838286	0.066715	0.051082	0.043917	90.23	7.24	2.14	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514240	M	M	NA	Agree	0.02	4.61	0.00	38.83	15.53	80.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867539	0.054652	0.05111	0.026699	92.04	5.50	2.09	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514241	M	M	NA	Agree	0.00	4.54	0.00	31.27	12.96	57.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85241	0.066207	0.05176	0.029623	91.89	5.84	1.91	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514242	M	M	NA	Agree	0.00	4.66	0.00	31.28	11.99	61.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860148	0.062159	0.051693	0.026001	92.01	5.59	2.03	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514243	M	M	NA	Agree	0.01	6.26	0.00	35.52	14.47	63.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86235	0.064613	0.050841	0.022196	92.54	5.09	2.02	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514244	M	M	NA	Agree	0.00	3.68	0.00	28.42	9.77	52.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870529	0.043542	0.051149	0.034781	91.22	6.31	2.14	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514245	M	M	NA	Agree	0.00	3.56	0.00	24.91	9.99	49.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875068	0.042302	0.051006	0.031624	91.03	6.09	2.55	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514246	M	M	NA	Agree	0.00	3.07	0.00	22.02	9.07	42.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865698	0.041608	0.051264	0.04143	90.11	6.97	2.59	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514247	M	M	NA	Agree	0.00	3.03	0.00	22.09	8.46	45.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860559	0.04546	0.05037	0.043611	89.92	7.26	2.48	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514248	M	M	NA	Agree	0.00	4.52	0.00	32.69	11.92	59.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881072	0.040068	0.052646	0.026213	91.47	5.53	2.66	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514249	M	M	NA	Agree	0.02	5.11	0.00	31.40	11.31	59.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863274	0.063174	0.052115	0.021438	92.35	5.19	2.07	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514250	M	M	NA	Agree	0.00	5.46	0.00	30.69	11.94	56.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862898	0.064847	0.05213	0.020126	92.67	5.00	1.95	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514251	M	M	NA	Agree	0.02	6.23	0.00	36.92	17.02	65.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858591	0.068324	0.052642	0.020443	92.53	4.85	2.25	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514252	M	M	NA	Agree	0.02	5.07	0.00	35.60	11.83	60.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.857032	0.069478	0.051391	0.0221	92.51	5.12	2.00	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514253	M	M	NA	Agree	0.00	4.85	0.00	31.79	11.84	58.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851146	0.071067	0.051323	0.026464	92.00	5.46	2.18	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514254	M	M	NA	Agree	0.04	4.38	0.00	30.29	11.09	61.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855387	0.065955	0.052095	0.026563	91.78	5.58	2.26	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514255	M	M	NA	Agree	0.03	4.65	0.00	31.27	10.73	56.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852107	0.073296	0.052403	0.022194	92.44	5.18	1.99	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514256	M	M	NA	Agree	0.05	4.55	0.00	32.26	11.33	56.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858252	0.071479	0.052409	0.01786	92.79	4.73	2.10	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514257	M	M	NA	Agree	0.00	3.72	0.00	31.52	9.78	48.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873612	0.042635	0.051609	0.032145	91.24	6.25	2.15	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514258	M	M	NA	Agree	0.01	3.34	0.00	28.64	10.17	49.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86994	0.043607	0.051701	0.034752	90.62	6.37	2.66	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514259	M	M	NA	Agree	0.00	3.37	0.00	26.03	8.61	52.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866642	0.043633	0.052699	0.037026	90.74	6.65	2.26	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514260	M	M	NA	Agree	0.00	3.29	0.00	24.92	9.46	43.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863814	0.043005	0.051544	0.041637	89.83	6.99	2.83	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514261	M	M	NA	Agree	0.01	2.66	0.00	20.83	9.53	47.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869952	0.041687	0.052109	0.036252	90.49	6.43	2.75	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2514262	M	M	NA	Agree	0.00	3.47	0.00	26.63	11.21	49.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879788	0.04222	0.052339	0.025653	92.01	5.43	2.22	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does