Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1584769	M	M	NA	Agree	0.13	3.32	0.00	32.19	10.69	55.72	NONE	POLYA	0.876376	0.053671	0.046128	0.023826	93.06	4.75	1.94	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584770	M	M	NA	Agree	0.05	4.85	0.00	33.36	11.00	56.59	NONE	POLYA	0.893947	0.040445	0.044769	0.020839	93.49	4.18	2.09	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584771	M	M	NA	Agree	0.07	3.61	0.00	26.39	10.15	53.87	NONE	POLYA	0.877414	0.051253	0.046368	0.024965	92.89	4.81	2.05	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584772	M	M	NA	Agree	0.03	5.75	0.00	31.47	12.62	61.74	NONE	POLYA	0.877795	0.060975	0.045721	0.015509	93.74	3.95	2.04	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584773	M	M	NA	Agree	0.04	3.12	0.00	27.29	11.29	70.12	NONE	POLYA	0.868988	0.047176	0.04738	0.036456	92.39	6.32	0.99	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584774	M	M	NA	Agree	0.01	5.34	0.00	28.54	13.10	64.56	NONE	POLYA	0.859876	0.057524	0.046976	0.035623	92.52	6.14	1.07	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584775	M	M	NA	Agree	0.06	4.00	0.00	28.53	11.50	59.92	NONE	POLYA	0.844605	0.069138	0.047496	0.038761	92.28	6.31	1.13	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584776	M	M	NA	Agree	0.00	4.19	0.00	27.04	11.74	60.09	NONE	POLYA	0.853864	0.054584	0.043341	0.048211	90.61	7.61	1.49	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584777	M	M	NA	Agree	0.05	3.87	0.00	29.82	9.02	68.46	NONE	POLYA	0.889951	0.037888	0.047544	0.024618	93.00	4.81	1.93	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584778	M	M	NA	Agree	0.04	4.31	0.00	29.80	9.98	61.45	NONE	POLYA	0.887322	0.037158	0.047549	0.02797	92.83	5.11	1.80	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584779	M	M	NA	Agree	0.03	3.83	0.00	29.20	10.77	59.40	NONE	POLYA	0.880803	0.042143	0.048989	0.028065	92.93	5.08	1.73	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584780	M	M	NA	Agree	0.05	4.59	0.00	33.36	10.50	56.49	NONE	POLYA	0.877553	0.052804	0.046678	0.022964	93.24	4.52	1.97	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584781	M	M	NA	Agree	0.05	3.69	0.00	29.38	11.41	72.75	NONE	POLYA	0.872039	0.042127	0.047574	0.03826	92.10	6.61	1.00	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584782	M	M	NA	Agree	0.10	5.53	0.00	33.71	13.31	71.81	NONE	POLYA	0.882065	0.042683	0.046927	0.028324	93.07	5.46	1.21	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584783	M	M	NA	Agree	0.04	4.30	0.00	30.74	12.43	61.33	NONE	POLYA	0.856413	0.056142	0.043541	0.043905	91.39	7.23	1.07	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1584784	M	M	NA	Agree	0.05	3.51	0.00	27.46	11.89	64.72	NONE	POLYA	0.872973	0.044212	0.043498	0.039318	91.60	6.83	1.29	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does