Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1532989	F	F	NA	Agree	197.04	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.853442	0.101168	0.042348	0.003042	92.83	4.18	2.47	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532990	F	F	NA	Agree	168.08	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.881554	0.080322	0.037042	0.001082	92.37	4.37	2.75	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532991	F	F	NA	Agree	221.05	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.864252	0.093807	0.040428	0.001512	92.89	4.28	2.35	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532992	F	F	NA	Agree	186.55	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.860375	0.093036	0.043679	0.00291	92.91	4.17	2.37	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532993	F	F	NA	Agree	183.42	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.866645	0.089291	0.042441	0.001623	92.98	4.08	2.40	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532994	F	F	NA	Agree	176.04	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.862013	0.093253	0.043442	0.001292	93.12	3.97	2.38	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532995	F	F	NA	Agree	171.15	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.870499	0.089679	0.037956	0.001865	92.46	4.71	2.32	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532996	F	F	NA	Agree	178.79	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.87533	0.087383	0.036524	0.000764	92.59	4.61	2.28	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532997	F	F	NA	Agree	213.28	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.859816	0.099484	0.038637	0.002063	92.51	4.66	2.30	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532998	F	F	NA	Agree	189.81	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.856069	0.099231	0.042678	0.002023	92.97	4.11	2.35	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1532999	F	F	NA	Agree	165.04	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.866566	0.091427	0.040511	0.001496	93.05	4.09	2.31	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1533000	F	F	NA	Agree	175.05	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.864339	0.093159	0.041192	0.00131	92.86	4.19	2.39	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does