Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1357492	M	M	NA	Agree	0.00	6.93	0.00	48.59	20.18	74.42	NONE	POLYA	0.849404	0.100764	0.045809	0.004023	95.12	2.41	2.15	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357493	M	M	NA	Agree	0.01	6.49	0.00	42.01	19.01	78.00	NONE	POLYA	0.858384	0.093735	0.043935	0.003946	95.47	2.34	1.89	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357494	M	M	NA	Agree	0.00	6.50	0.00	47.16	20.79	79.76	NONE	POLYA	0.857204	0.094239	0.045063	0.003495	95.09	2.33	2.27	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357495	M	M	NA	Agree	0.00	5.26	0.00	40.51	19.04	75.66	NONE	POLYA	0.858423	0.093708	0.045194	0.002675	95.30	2.30	2.10	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357496	M	M	NA	Agree	0.00	6.31	0.00	44.34	17.44	78.76	NONE	POLYA	0.855839	0.095287	0.045168	0.003706	95.30	2.34	2.06	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357497	M	M	NA	Agree	0.00	6.31	0.00	46.51	16.41	84.25	NONE	POLYA	0.853582	0.090414	0.046996	0.009008	94.54	2.96	2.20	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357498	M	M	NA	Agree	0.00	5.47	0.00	43.57	16.39	83.87	NONE	POLYA	0.855308	0.094459	0.046006	0.004227	94.98	2.45	2.31	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357499	M	M	NA	Agree	0.00	5.71	0.00	47.58	19.65	77.95	NONE	POLYA	0.853538	0.097739	0.045386	0.003337	95.07	2.31	2.30	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357500	M	M	NA	Agree	0.05	5.80	0.00	42.91	20.44	76.27	NONE	POLYA	0.847515	0.102007	0.047859	0.002619	95.03	2.39	2.24	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357501	M	M	NA	Agree	0.00	6.94	0.00	48.61	17.33	79.54	NONE	POLYA	0.836161	0.101057	0.044971	0.017812	93.82	3.78	2.07	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357502	M	M	NA	Agree	0.00	5.75	0.00	50.69	21.46	73.22	NONE	POLYA	0.84023	0.105505	0.046808	0.007457	94.88	2.88	1.92	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357503	M	M	NA	Agree	0.02	6.14	0.00	46.19	17.31	79.91	NONE	POLYA	0.845564	0.104149	0.046172	0.004115	94.83	2.50	2.35	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1357504	M	M	NA	Agree	0.00	6.88	0.00	47.57	20.70	74.70	NONE	POLYA	0.847141	0.104778	0.045043	0.003038	95.28	2.32	2.09	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does