Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1055025	M	M	NA	Agree	0.15	4.89	0.00	29.64	10.85	29.64	NONE	POLYA	0.865021	0.092992	0.023557	0.018431	90.66	5.93	2.85	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055026	M	M	NA	Agree	0.13	4.72	0.00	31.79	10.61	26.79	NONE	POLYA	0.860642	0.097859	0.024896	0.016603	90.97	5.93	2.52	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055027	M	M	NA	Agree	0.10	5.59	0.00	34.63	13.37	26.66	NONE	POLYA	0.860981	0.097306	0.026097	0.015616	91.58	5.34	2.53	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055075	M	M	NA	Agree	0.00	7.38	0.00	30.75	12.17	66.58	NONE	POLYA	0.903178	0.044876	0.034687	0.017259	91.12	5.78	2.55	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055076	M	M	NA	Agree	0.03	7.94	0.00	34.57	14.53	72.51	NONE	POLYA	0.895285	0.05736	0.037539	0.009816	91.92	5.04	2.47	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055077	M	M	NA	Agree	0.04	6.26	0.00	29.45	15.14	70.36	NONE	POLYA	0.900455	0.054507	0.037307	0.007731	92.27	4.70	2.49	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055120	M	M	NA	Agree	0.14	6.62	0.00	40.12	17.34	66.83	NONE	POLYA	0.86249	0.08101	0.048057	0.008444	93.47	3.52	2.61	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055121	M	M	NA	Agree	0.08	6.39	0.00	51.77	26.73	59.56	NONE	POLYA	0.80171	0.142439	0.050894	0.004957	94.05	2.86	2.58	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1055122	M	M	NA	Agree	0.00	7.18	0.00	41.70	19.75	58.91	NONE	POLYA	0.839655	0.105114	0.049998	0.005233	93.98	3.14	2.46	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does