Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1020666	M	M	NA	Agree	0.06	6.00	0.00	41.40	18.80	52.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.811699	0.133692	0.047725	0.006884	94.25	2.65	2.69	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020667	M	M	NA	Agree	1.21	7.28	0.00	23.62	19.60	59.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.735485	0.178393	0.050776	0.035345	86.37	8.25	4.61	216	LP2 (2026-09-30)	PASS	NonPolyA_perM 216 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020668	M	M	NA	Agree	0.01	0.75	0.00	6.73	3.39	27.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.829583	0.0812	0.029409	0.059808	85.14	9.80	4.43	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020669	M	M	NA	Agree	0.04	5.47	0.00	25.17	7.38	51.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886232	0.054794	0.038846	0.020129	91.05	6.33	1.93	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020670	M	M	NA	Agree	0.10	1.71	0.00	17.54	5.98	22.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886047	0.06739	0.026116	0.020447	87.97	7.87	3.21	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020671	M	M	NA	Agree	0.39	6.85	0.00	35.59	24.13	51.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.78824	0.145572	0.049168	0.01702	91.05	5.84	2.21	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020672	M	M	NA	Agree	0.01	1.58	0.00	37.26	4.37	34.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895829	0.048577	0.025788	0.029807	88.03	7.29	3.86	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020673	M	M	NA	Agree	0.56	7.29	0.00	34.62	20.02	45.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.738583	0.188438	0.042252	0.030727	86.58	10.26	2.42	260	LP2 (2026-09-30)	PASS	NonPolyA_perM 260 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020674	M	M	NA	Agree	0.05	0.79	0.00	2.42	0.59	10.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.780988	0.02502	0.027885	0.166107	78.17	19.27	1.95	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020675	M	M	NA	Agree	0.05	5.98	0.00	40.33	16.36	54.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.808853	0.129122	0.049311	0.012713	92.71	3.31	3.60	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020676	M	M	NA	Agree	0.50	6.65	0.00	23.28	19.98	49.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.804963	0.12515	0.037653	0.032234	91.60	6.27	1.76	188	LP2 (2026-09-30)	PASS	NonPolyA_perM 188 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020677	M	M	NA	Agree	0.01	2.53	0.00	18.60	6.04	26.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874344	0.069577	0.02166	0.03442	91.62	5.91	2.18	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020678	M	M	NA	Agree	0.05	4.63	0.00	20.85	10.94	40.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851558	0.088418	0.039091	0.020933	93.13	5.13	1.28	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020679	M	M	NA	Agree	0.07	1.36	0.00	15.81	5.61	18.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884327	0.072976	0.019195	0.023503	93.05	5.34	1.34	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020680	M	M	NA	Agree	0.11	6.52	0.00	34.49	31.29	52.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.790639	0.155911	0.041253	0.012197	93.63	3.75	2.12	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020681	M	M	NA	Agree	0.00	2.30	0.00	26.42	5.01	24.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909296	0.045588	0.018485	0.02663	92.63	4.74	2.41	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020682	M	M	NA	Agree	0.34	4.87	0.00	24.48	16.18	35.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.762939	0.170398	0.03693	0.029733	90.14	8.26	1.21	210	LP2 (2026-09-30)	PASS	NonPolyA_perM 210 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020683	M	M	NA	Agree	0.09	1.30	0.00	1.60	0.60	19.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.830093	0.094756	0.055726	0.019425	92.89	4.86	1.20	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020684	M	M	NA	Agree	0.03	6.78	0.00	43.38	15.15	51.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.807216	0.129049	0.050512	0.013223	90.94	4.42	3.87	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020685	M	M	NA	Agree	0.45	4.56	0.00	23.64	17.25	60.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.803798	0.123542	0.048561	0.024099	86.05	7.71	4.94	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020686	M	M	NA	Agree	0.02	2.34	0.00	14.20	4.00	34.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.836459	0.091604	0.025521	0.046415	87.89	8.36	3.08	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020687	M	M	NA	Agree	0.15	1.91	0.00	12.86	7.83	38.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.836231	0.099429	0.043202	0.021137	89.18	6.28	3.50	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020688	M	M	NA	Agree	0.13	1.84	0.00	16.84	6.06	32.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861461	0.089906	0.031662	0.01697	87.68	7.54	3.71	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020689	M	M	NA	Agree	0.13	8.90	0.00	43.80	24.42	63.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.757976	0.176009	0.048731	0.017285	87.85	6.07	4.65	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020690	M	M	NA	Agree	0.00	0.15	0.00	2.38	1.17	5.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940159	0.027898	0.020915	0.011028	90.25	5.35	3.62	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020691	M	M	NA	Agree	0.27	10.34	0.00	43.01	19.24	81.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.767916	0.146349	0.046714	0.039021	82.66	12.51	3.57	272	LP2 (2026-09-30)	PASS	NonPolyA_perM 272 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1020692	M	M	NA	Agree	0.04	0.72	0.00	1.46	0.50	11.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884434	0.032353	0.033185	0.050027	87.04	8.73	3.46	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does