Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9608208	F	M	NA	Conflict	0.03	5.41	0.00	43.10	10.31	64.78	NONE	POLYA	0.912611	0.048287	0.037629	0.001473	96.50	1.89	1.43	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608209	F	M	NA	Conflict	0.03	5.30	0.00	37.51	10.80	59.77	NONE	POLYA	0.911786	0.051518	0.035061	0.001635	96.43	1.93	1.45	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608210	F	M	NA	Conflict	0.02	3.46	0.00	32.19	9.82	51.50	NONE	POLYA	0.905999	0.05617	0.036471	0.00136	96.39	1.92	1.50	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608211	F	M	NA	Conflict	0.01	3.01	0.00	29.05	10.10	65.75	NONE	POLYA	0.903067	0.059091	0.036349	0.001493	96.47	2.07	1.25	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608212	F	M	NA	Conflict	0.02	3.11	0.00	28.77	7.51	46.20	NONE	POLYA	0.913126	0.050417	0.03524	0.001217	96.28	2.15	1.36	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608213	F	M	NA	Conflict	0.04	4.14	0.00	34.62	8.08	51.19	NONE	POLYA	0.908827	0.05425	0.035849	0.001074	96.43	1.88	1.48	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608214	F	M	NA	Conflict	0.03	6.28	0.00	41.30	12.84	64.34	NONE	POLYA	0.901819	0.056702	0.040049	0.00143	96.40	1.94	1.48	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608215	F	M	NA	Conflict	0.12	5.46	0.00	30.73	10.05	41.38	NONE	POLYA	0.911681	0.051046	0.035854	0.001418	96.70	1.88	1.23	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608216	F	M	NA	Conflict	0.05	7.68	0.00	44.59	12.75	77.08	NONE	POLYA	0.88998	0.067707	0.04091	0.001403	96.34	2.19	1.25	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608217	F	M	NA	Conflict	0.04	5.52	0.00	33.50	12.44	52.39	NONE	POLYA	0.893588	0.066836	0.03792	0.001657	96.72	1.80	1.29	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608218	F	M	NA	Conflict	0.05	4.11	0.00	27.12	7.37	47.55	NONE	POLYA	0.91932	0.044034	0.035295	0.001351	95.86	2.47	1.44	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608219	F	M	NA	Conflict	0.05	6.15	0.00	41.19	10.08	56.42	NONE	POLYA	0.905412	0.057804	0.035447	0.001338	96.11	2.18	1.50	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608220	F	M	NA	Conflict	0.06	4.66	0.00	33.67	9.16	75.40	NONE	POLYA	0.899727	0.062636	0.03632	0.001317	96.09	2.08	1.68	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608221	F	M	NA	Conflict	0.05	4.25	0.00	26.95	7.44	63.61	NONE	POLYA	0.903432	0.058679	0.035984	0.001906	96.11	2.10	1.66	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608222	F	M	NA	Conflict	0.03	4.44	0.00	26.30	8.13	56.74	NONE	POLYA	0.909722	0.053403	0.035323	0.001553	96.40	1.97	1.49	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608223	F	M	NA	Conflict	0.04	2.53	0.00	18.69	7.12	49.39	NONE	POLYA	0.91815	0.044606	0.035893	0.001351	96.13	2.12	1.60	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608224	F	M	NA	Conflict	0.05	4.11	0.00	26.00	6.55	51.44	NONE	POLYA	0.917547	0.046214	0.035044	0.001196	96.07	2.23	1.54	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608225	F	M	NA	Conflict	0.05	3.74	0.00	28.04	8.23	63.18	NONE	POLYA	0.901652	0.059654	0.037386	0.001308	96.02	2.17	1.66	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608226	F	M	NA	Conflict	0.01	6.85	0.00	45.81	13.09	67.12	NONE	POLYA	0.86397	0.084855	0.043805	0.007371	95.56	2.50	1.76	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608227	F	M	NA	Conflict	0.02	3.54	0.00	21.92	8.21	56.48	NONE	POLYA	0.891429	0.064595	0.040761	0.003216	96.09	2.21	1.54	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608228	F	M	NA	Conflict	0.04	6.10	0.00	38.69	11.95	70.59	NONE	POLYA	0.86741	0.083747	0.045469	0.003373	95.98	2.20	1.62	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608229	F	M	NA	Conflict	0.03	2.84	0.00	21.38	8.28	53.51	NONE	POLYA	0.894773	0.063123	0.040006	0.002097	95.52	2.91	1.39	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608230	F	M	NA	Conflict	0.00	6.20	0.00	35.84	8.02	53.50	NONE	POLYA	0.881792	0.074052	0.04246	0.001696	94.67	3.40	1.67	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9608231	F	M	NA	Conflict	0.02	4.38	0.00	29.59	9.33	53.94	NONE	POLYA	0.877361	0.077219	0.04266	0.00276	95.11	3.19	1.48	158	LP2 (2026-09-30)	PASS	NonPolyA_perM 158 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does