Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9381452	M	M	NA	Agree	0.00	4.13	0.00	34.31	8.94	52.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887674	0.084171	0.027865	0.000291	97.09	2.85	0.05	192	LP2 (2026-09-30)	PASS	NonPolyA_perM 192 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9381453	M	M	NA	Agree	0.00	3.79	0.00	31.69	6.61	46.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900268	0.071744	0.027931	0.000057	97.02	2.88	0.08	224	LP2 (2026-09-30)	PASS	NonPolyA_perM 224 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9381454	M	M	NA	Agree	0.03	3.84	0.00	40.71	9.57	48.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868855	0.096723	0.034159	0.000263	96.44	3.47	0.07	152	LP2 (2026-09-30)	PASS	NonPolyA_perM 152 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9381455	M	M	NA	Agree	0.02	4.38	0.00	35.80	8.43	44.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886607	0.082868	0.030211	0.000314	97.01	2.88	0.09	155	LP2 (2026-09-30)	PASS	NonPolyA_perM 155 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9381456	M	M	NA	Agree	0.00	3.90	0.00	27.73	9.30	52.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.892446	0.073856	0.033124	0.000575	96.17	3.67	0.14	243	LP2 (2026-09-30)	PASS	NonPolyA_perM 243 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9381457	M	M	NA	Agree	0.02	4.70	0.00	24.73	7.69	47.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900673	0.068023	0.031251	0.000053	96.09	3.76	0.12	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9381458	M	M	NA	Agree	0.00	4.53	0.00	26.11	9.38	46.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890303	0.078012	0.031404	0.000281	96.86	3.01	0.11	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9381459	M	M	NA	Agree	0.00	4.72	0.00	26.53	9.90	44.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884589	0.081746	0.033287	0.000378	96.21	3.64	0.13	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does