Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-11-04) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9090858	M	M	NA	Agree	0.01	3.09	0.00	25.86	4.74	36.01	NONE	POLYA	0.907767	0.049097	0.021875	0.021261	88.66	3.24	7.91	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090859	M	M	NA	Agree	0.00	3.44	0.00	33.20	5.95	47.17	NONE	POLYA	0.904649	0.048253	0.022528	0.02457	91.25	3.48	5.09	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090860	M	M	NA	Agree	0.00	3.87	0.00	36.99	7.05	53.71	NONE	POLYA	0.90281	0.052834	0.024	0.020355	90.93	3.42	5.39	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090861	M	M	NA	Agree	0.01	2.49	0.00	22.07	3.39	33.70	NONE	POLYA	0.914552	0.044654	0.01921	0.021584	91.18	3.26	5.32	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090862	M	M	NA	Agree	0.00	2.13	0.00	13.87	5.90	21.01	NONE	POLYA	0.894283	0.064692	0.024912	0.016112	91.29	2.92	5.58	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090863	M	M	NA	Agree	0.00	1.93	0.00	21.93	6.01	28.41	NONE	POLYA	0.883872	0.067903	0.030343	0.017882	91.05	3.26	5.36	152	LP2 (2026-09-30)	PASS	NonPolyA_perM 152 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090864	M	M	NA	Agree	0.00	3.95	0.00	25.16	6.52	43.49	NONE	POLYA	0.898712	0.058569	0.023728	0.018992	79.05	2.70	18.12	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090865	M	M	NA	Agree	0.00	2.20	0.00	22.80	4.66	38.81	NONE	POLYA	0.882768	0.040541	0.021599	0.055093	76.76	5.80	17.29	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090866	M	M	NA	Agree	0.00	2.76	0.00	20.96	5.35	31.81	NONE	POLYA	0.90684	0.047686	0.021866	0.023607	80.49	3.38	15.96	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090867	M	M	NA	Agree	0.05	4.22	0.00	24.72	8.60	54.12	NONE	POLYA	0.857308	0.059552	0.025175	0.057965	78.32	6.31	15.20	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090868	M	M	NA	Agree	0.04	2.66	0.00	27.34	5.15	42.51	NONE	POLYA	0.924215	0.040865	0.023816	0.011103	83.25	2.19	14.39	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090869	M	M	NA	Agree	0.04	5.02	0.00	39.54	9.30	53.09	NONE	POLYA	0.825413	0.074593	0.026701	0.073294	88.97	8.15	2.74	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090870	M	M	NA	Agree	0.03	4.09	0.00	33.42	9.45	51.12	NONE	POLYA	0.823034	0.088048	0.024346	0.064572	89.49	7.16	3.20	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090871	M	M	NA	Agree	0.03	4.51	0.00	29.40	9.08	47.90	NONE	POLYA	0.791118	0.094971	0.024493	0.089417	87.86	9.49	2.48	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090872	M	M	NA	Agree	0.01	4.72	0.00	32.55	8.53	58.80	NONE	POLYA	0.881132	0.069137	0.026734	0.022996	90.55	3.71	5.53	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090873	M	M	NA	Agree	0.06	5.69	0.00	33.42	11.74	61.66	NONE	POLYA	0.846444	0.098547	0.030788	0.024221	90.31	3.99	5.44	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090874	M	M	NA	Agree	0.03	4.89	0.00	29.67	8.87	67.21	NONE	POLYA	0.890604	0.061433	0.030352	0.017611	90.43	3.42	5.94	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090875	M	M	NA	Agree	0.01	3.03	0.00	29.72	6.21	45.35	NONE	POLYA	0.901483	0.04837	0.022885	0.027262	90.80	3.77	5.29	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090876	M	M	NA	Agree	0.01	4.14	0.00	34.90	7.63	45.51	NONE	POLYA	0.830833	0.065607	0.024046	0.079514	89.10	8.18	2.55	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090877	M	M	NA	Agree	0.00	2.92	0.00	32.18	5.87	42.35	NONE	POLYA	0.805664	0.074181	0.023731	0.096424	87.09	10.11	2.64	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090878	M	M	NA	Agree	0.00	3.21	0.00	24.86	4.70	38.09	NONE	POLYA	0.7808	0.077221	0.025854	0.116125	84.99	11.72	3.11	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090879	M	M	NA	Agree	0.00	2.86	0.00	24.00	4.95	40.44	NONE	POLYA	0.905252	0.042705	0.023842	0.0282	90.98	3.88	4.97	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090880	M	M	NA	Agree	0.00	1.54	0.00	22.24	4.24	31.44	NONE	POLYA	0.917473	0.041659	0.019695	0.021173	90.74	3.04	6.10	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090881	M	M	NA	Agree	0.04	3.20	0.00	26.88	5.47	41.21	NONE	POLYA	0.909864	0.045505	0.021502	0.023129	78.59	3.07	18.21	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090882	M	M	NA	Agree	0.00	2.49	0.00	19.21	4.15	33.73	NONE	POLYA	0.896234	0.048246	0.021091	0.034429	83.95	4.11	11.83	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090883	M	M	NA	Agree	0.08	1.67	0.00	15.07	5.08	29.75	NONE	POLYA	0.91332	0.042621	0.021175	0.022884	81.11	3.27	15.49	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090884	M	M	NA	Agree	0.00	2.31	0.00	28.69	4.08	41.02	NONE	POLYA	0.914515	0.0345	0.020976	0.030009	81.80	3.70	14.33	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090885	M	M	NA	Agree	0.01	3.23	0.00	22.92	5.09	37.95	NONE	POLYA	0.912143	0.046287	0.020038	0.021532	80.87	3.08	15.88	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090886	M	M	NA	Agree	0.03	3.45	0.00	33.15	6.79	45.95	NONE	POLYA	0.799845	0.069555	0.02125	0.10935	85.73	11.14	2.99	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090887	M	M	NA	Agree	0.00	4.25	0.00	29.85	6.55	44.38	NONE	POLYA	0.831905	0.062986	0.021679	0.083431	88.75	8.66	2.43	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090888	M	M	NA	Agree	0.06	3.74	0.00	33.84	7.75	38.97	NONE	POLYA	0.826615	0.062546	0.021548	0.089291	87.72	9.07	3.01	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090889	M	M	NA	Agree	0.00	2.28	0.00	19.49	6.88	30.76	NONE	POLYA	0.910569	0.047632	0.022349	0.019451	67.32	2.40	30.08	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090890	M	M	NA	Agree	0.00	1.59	0.00	18.75	3.40	32.84	NONE	POLYA	0.936639	0.031335	0.019071	0.012955	91.72	2.42	5.60	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090891	M	M	NA	Agree	0.00	2.57	0.00	19.32	5.02	40.69	NONE	POLYA	0.921898	0.040741	0.022131	0.015231	91.48	2.75	5.57	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090892	M	M	NA	Agree	0.00	1.80	0.00	16.54	4.01	34.07	NONE	POLYA	0.819463	0.05252	0.020299	0.107718	86.52	10.99	2.38	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090893	M	M	NA	Agree	0.00	2.36	0.00	27.20	4.57	33.38	NONE	POLYA	0.843815	0.053613	0.020585	0.081987	88.12	8.61	3.14	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090894	M	M	NA	Agree	0.01	3.17	0.00	33.09	5.72	34.15	NONE	POLYA	0.919104	0.035167	0.018471	0.027258	88.29	3.51	8.11	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090895	M	M	NA	Agree	0.00	1.83	0.00	21.74	4.96	34.47	NONE	POLYA	0.899207	0.072567	0.023823	0.004404	94.90	1.86	3.12	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090896	M	M	NA	Agree	0.00	1.46	0.00	9.91	3.00	26.96	NONE	POLYA	0.923176	0.036853	0.018054	0.021916	88.87	3.28	7.68	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9090897	M	M	NA	Agree	0.00	1.23	0.00	10.56	4.71	14.52	NONE	POLYA	0.921076	0.046013	0.018273	0.014638	90.64	2.78	6.33	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does