Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 1 sample processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9074624	F	F	NA	Agree	76.65	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.923869	0.02784	0.034705	0.013586	90.21	2.96	6.68	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074625	F	F	NA	Agree	53.19	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.933967	0.025048	0.032789	0.008195	92.73	2.64	4.47	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074626	F	F	NA	Agree	40.21	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.928287	0.024528	0.029594	0.017591	90.71	3.34	5.79	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074627	F	F	NA	Agree	43.76	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.933151	0.024596	0.03201	0.010243	90.98	2.89	5.96	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074628	F	F	NA	Agree	71.05	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.91622	0.031058	0.032479	0.020243	91.45	3.52	4.87	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074629	F	F	NA	Agree	0.00	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.89524	0.03528	0.03409	0.03539	88.73	5.12	5.98	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074630	F	F	NA	Agree	20.03	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.925303	0.019615	0.035757	0.019325	91.34	4.22	4.30	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074631	F	F	NA	Agree	70.33	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.915184	0.031185	0.037835	0.015796	92.05	3.20	4.54	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9074632	F	F	NA	Agree	133.98	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.906013	0.042531	0.035417	0.016039	88.43	3.24	8.18	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does