Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9026255	M	M	NA	Agree	0.00	6.14	0.00	36.72	15.54	66.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837197	0.111417	0.04832	0.003066	90.75	1.65	7.40	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026256	M	M	NA	Agree	0.07	7.49	0.00	35.89	13.67	72.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.832578	0.116501	0.047805	0.003115	92.19	1.60	6.02	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026257	M	M	NA	Agree	0.10	7.28	0.00	35.75	13.96	69.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837831	0.111548	0.047555	0.003066	92.78	1.65	5.37	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026258	M	M	NA	Agree	0.03	7.01	0.00	36.66	13.67	70.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826397	0.122632	0.04792	0.003052	92.36	1.61	5.83	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026259	M	M	NA	Agree	0.00	5.50	0.00	34.75	12.53	60.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843856	0.109055	0.044212	0.002878	92.84	1.52	5.44	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026260	M	F	NA	Conflict	155.72	0.00	0.00	0.17	13.21	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.840674	0.109032	0.04803	0.002265	93.07	1.52	5.23	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026261	M	M	NA	Agree	0.06	7.33	0.00	41.14	12.65	75.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838499	0.110503	0.047847	0.00315	92.70	1.63	5.47	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026262	M	M	NA	Agree	0.03	6.50	0.00	42.50	16.28	80.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845259	0.104821	0.047558	0.002363	93.05	1.56	5.19	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026263	M	M	NA	Agree	0.10	6.49	0.00	32.20	11.51	69.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.817059	0.131753	0.047384	0.003804	92.24	1.91	5.63	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026264	M	M	NA	Agree	0.07	7.49	0.00	38.11	12.73	82.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826549	0.121275	0.049091	0.003085	92.97	1.92	4.87	140	LP2 (2026-09-30)	PASS	NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026265	M	M	NA	Agree	0.03	6.91	0.00	38.86	16.19	72.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.828987	0.121385	0.046589	0.003039	93.12	1.59	5.07	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026266	M	M	NA	Agree	0.04	8.24	0.00	33.68	13.95	73.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.819662	0.130966	0.046367	0.003005	91.31	1.79	6.68	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026267	M	M	NA	Agree	0.03	7.31	0.00	31.01	12.19	66.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.821756	0.12755	0.047357	0.003337	91.52	1.80	6.46	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026268	M	M	NA	Agree	0.03	7.24	0.00	32.88	10.77	65.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.836223	0.117115	0.043458	0.003205	92.83	1.92	5.05	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026269	M	M	NA	Agree	0.00	6.96	0.00	28.88	11.20	70.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849033	0.103064	0.045051	0.002853	92.46	1.72	5.62	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026270	M	M	NA	Agree	0.00	8.00	0.00	34.83	16.04	67.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.819145	0.130237	0.045805	0.004813	92.44	1.78	5.58	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026271	M	M	NA	Agree	0.06	5.66	0.00	30.58	11.01	72.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852039	0.099738	0.045352	0.002871	93.06	1.70	5.06	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026272	M	M	NA	Agree	0.00	7.06	0.00	30.00	14.22	78.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.842999	0.108502	0.044857	0.003641	93.00	1.72	5.09	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026273	M	M	NA	Agree	0.09	8.22	0.00	37.30	14.08	80.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.831724	0.1183	0.047561	0.002415	93.74	1.73	4.32	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026274	M	M	NA	Agree	0.02	7.45	0.00	30.51	11.78	86.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.82376	0.125472	0.046676	0.004091	93.25	1.77	4.77	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026275	M	M	NA	Agree	0.00	5.32	0.00	30.59	12.64	86.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826418	0.124243	0.046552	0.002787	92.35	1.70	5.75	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026276	M	M	NA	Agree	0.04	8.54	0.00	39.21	14.78	81.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.814443	0.133453	0.048879	0.003225	92.89	1.83	5.04	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026277	M	M	NA	Agree	0.02	5.37	0.00	27.41	10.88	80.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845226	0.106555	0.045151	0.003068	92.91	1.86	5.04	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9026278	M	M	NA	Agree	0.03	5.84	0.00	38.79	11.74	78.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.84239	0.109142	0.045911	0.002556	92.22	1.85	5.71	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does