Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). SampleID InputSex ComputedSex XYRatio Agreement Xist Uty Sry Ddx3y Kdm5d Eif2s3y ComputedStrand ComputedLibraryPrep PctMRNA PctIntronic PctIntergenic PctRibosomal UniqPct MultiPct TooShortPct NonPolyA_perM LibraryPrepRule LibraryPrepQC LibraryPrepReason GSM8994145 M M NA Agree 0.13 3.33 0.00 14.95 10.30 45.69 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.852151 0.053861 0.034974 0.059015 89.96 7.82 2.05 107 LP2 (2026-09-30) PASS NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994146 F F NA Agree 32.97 0.05 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.853838 0.04954 0.033638 0.062984 90.24 7.85 1.76 87 LP2 (2026-09-30) PASS NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994147 M M NA Agree 0.10 3.87 0.00 18.09 11.96 53.22 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.856937 0.056147 0.036383 0.050533 91.79 6.58 1.49 97 LP2 (2026-09-30) PASS NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994148 F F NA Agree 45.10 0.00 0.00 0.00 0.00 0.05 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.852529 0.053785 0.036769 0.056917 90.53 7.16 2.17 92 LP2 (2026-09-30) PASS NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994149 M M NA Agree 0.00 3.85 0.00 15.78 12.65 50.85 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.840425 0.058752 0.036181 0.064643 89.22 8.44 2.18 117 LP2 (2026-09-30) PASS NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994150 F F NA Agree 46.20 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.913014 0.048262 0.03469 0.004035 95.58 2.18 2.09 122 LP2 (2026-09-30) PASS NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994151 M M NA Agree 0.56 3.25 0.00 13.52 9.00 51.13 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.889046 0.059669 0.048591 0.002694 95.60 1.98 2.25 90 LP2 (2026-09-30) PASS NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994152 F F NA Agree 54.45 0.00 0.00 0.02 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908873 0.051225 0.037415 0.002487 95.89 2.06 1.90 104 LP2 (2026-09-30) PASS NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994153 M M NA Agree 0.03 3.09 0.00 17.61 11.27 65.44 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.904531 0.05003 0.035365 0.010074 95.24 2.84 1.76 82 LP2 (2026-09-30) PASS NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994154 M M NA Agree 0.05 3.86 0.00 16.21 11.55 44.39 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908981 0.05185 0.036247 0.002922 95.82 2.16 1.88 128 LP2 (2026-09-30) PASS NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994155 F F NA Agree 40.59 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.911323 0.048309 0.038305 0.002063 96.18 1.90 1.77 106 LP2 (2026-09-30) PASS NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994156 M M NA Agree 1.02 3.33 0.00 17.16 12.04 55.18 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.913431 0.04791 0.037086 0.001573 95.89 1.95 2.00 104 LP2 (2026-09-30) PASS NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994157 F F NA Agree 49.84 0.00 0.00 0.00 0.00 0.17 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.901434 0.053093 0.036093 0.009379 95.09 2.84 1.92 100 LP2 (2026-09-30) PASS NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994158 F F NA Agree 47.85 0.00 0.00 0.12 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.905536 0.052296 0.040515 0.001653 96.03 1.88 1.94 94 LP2 (2026-09-30) PASS NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994159 M M NA Agree 0.00 4.88 0.00 20.77 12.30 67.17 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908098 0.05002 0.039257 0.002625 96.06 1.88 1.93 92 LP2 (2026-09-30) PASS NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994160 F F NA Agree 39.59 0.00 0.00 0.00 0.00 0.28 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908882 0.050354 0.037161 0.003603 96.31 1.90 1.64 78 LP2 (2026-09-30) PASS NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994161 M M NA Agree 0.00 3.21 0.00 11.39 11.71 54.50 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.909024 0.047875 0.039148 0.003953 95.81 2.17 1.86 91 LP2 (2026-09-30) PASS NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994162 M M NA Agree 0.02 3.37 0.00 12.24 12.35 57.25 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.910306 0.048423 0.039564 0.001707 95.95 1.89 2.00 103 LP2 (2026-09-30) PASS NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994163 F F NA Agree 47.34 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.909252 0.049878 0.03764 0.003229 95.92 2.20 1.72 96 LP2 (2026-09-30) PASS NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994164 M F NA Conflict 42.65 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.913539 0.049723 0.032124 0.004614 96.04 2.04 1.78 81 LP2 (2026-09-30) PASS NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994165 F F NA Agree 42.63 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.912041 0.04852 0.036054 0.003385 96.07 2.10 1.69 88 LP2 (2026-09-30) PASS NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994166 F F NA Agree 48.29 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.910983 0.052883 0.030262 0.005871 95.59 2.34 1.93 92 LP2 (2026-09-30) PASS NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994167 F F NA Agree 44.21 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.911343 0.048095 0.027328 0.013235 95.02 3.06 1.80 80 LP2 (2026-09-30) PASS NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994168 F M NA Conflict 0.20 3.67 0.00 20.14 11.23 46.65 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.911555 0.050386 0.034389 0.003669 96.08 1.93 1.85 75 LP2 (2026-09-30) PASS NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994169 M M NA Agree 0.09 3.65 0.00 15.56 8.61 49.60 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.915982 0.047319 0.032127 0.004572 95.62 2.32 1.93 87 LP2 (2026-09-30) PASS NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994170 M M NA Agree 0.00 3.07 0.00 18.61 9.41 45.72 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.911279 0.050978 0.032327 0.005416 95.71 2.33 1.83 81 LP2 (2026-09-30) PASS NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994171 F F NA Agree 45.70 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.909539 0.050955 0.034822 0.004684 95.85 2.25 1.77 95 LP2 (2026-09-30) PASS NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994172 M M NA Agree 0.01 3.27 0.00 20.92 10.48 47.28 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908359 0.053099 0.034717 0.003825 95.99 1.95 1.91 72 LP2 (2026-09-30) PASS NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994173 F F NA Agree 43.74 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.912096 0.050063 0.03352 0.004322 95.44 2.29 2.12 92 LP2 (2026-09-30) PASS NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994174 F F NA Agree 51.98 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908887 0.055504 0.03045 0.00516 95.90 2.26 1.71 109 LP2 (2026-09-30) PASS NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994175 M M NA Agree 0.01 3.97 0.00 23.33 11.52 42.67 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.905954 0.05666 0.027265 0.010121 94.68 3.35 1.79 111 LP2 (2026-09-30) PASS NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994176 F M NA Conflict 0.01 2.88 0.00 19.50 10.51 48.74 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.906327 0.051649 0.034876 0.007147 95.50 2.65 1.70 84 LP2 (2026-09-30) PASS NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994177 M M NA Agree 0.01 2.98 0.00 20.29 10.42 44.92 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.915603 0.047742 0.032074 0.00458 96.11 2.18 1.57 91 LP2 (2026-09-30) PASS NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994178 F F NA Agree 32.60 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.916019 0.04755 0.03266 0.003771 96.17 2.08 1.60 88 LP2 (2026-09-30) PASS NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994179 M M NA Agree 0.00 4.93 0.00 26.64 11.94 53.46 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.903736 0.051918 0.034341 0.010005 95.05 3.12 1.67 108 LP2 (2026-09-30) PASS NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994180 F F NA Agree 44.54 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.890495 0.056497 0.029521 0.023486 92.80 5.27 1.75 127 LP2 (2026-09-30) PASS NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994181 M M NA Agree 0.01 2.53 0.00 23.71 10.65 48.47 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908986 0.047775 0.034091 0.009147 90.33 2.61 6.92 104 LP2 (2026-09-30) PASS NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994182 M M NA Agree 0.12 3.61 0.00 34.27 11.40 54.46 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.891175 0.059445 0.032825 0.016555 92.41 2.99 4.47 103 LP2 (2026-09-30) PASS NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994183 F F NA Agree 18.50 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.899238 0.048821 0.032434 0.019507 94.73 3.45 1.71 74 LP2 (2026-09-30) PASS NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994184 F F NA Agree 55.60 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.896657 0.055039 0.032785 0.01552 95.15 3.07 1.64 81 LP2 (2026-09-30) PASS NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994185 M M NA Agree 0.05 3.77 0.00 25.71 10.59 47.86 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.891167 0.052536 0.031455 0.024842 93.71 3.97 2.20 84 LP2 (2026-09-30) PASS NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994186 F F NA Agree 62.91 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.892464 0.058007 0.033594 0.015934 95.07 3.10 1.70 91 LP2 (2026-09-30) PASS NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994187 F F NA Agree 54.71 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.888443 0.055189 0.032567 0.023801 94.11 4.00 1.75 82 LP2 (2026-09-30) PASS NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994188 F F NA Agree 56.34 0.00 0.00 0.00 0.09 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.892377 0.057944 0.033814 0.015865 95.28 2.96 1.64 80 LP2 (2026-09-30) PASS NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994189 F F NA Agree 56.23 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.889043 0.051221 0.033983 0.025753 93.51 4.10 2.26 99 LP2 (2026-09-30) PASS NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994190 F F NA Agree 54.06 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.895042 0.050665 0.031563 0.02273 94.09 3.72 2.07 79 LP2 (2026-09-30) PASS NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994191 F M NA Conflict 0.00 2.34 0.00 26.07 10.63 50.78 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.899296 0.047818 0.031793 0.021093 87.43 3.34 9.10 101 LP2 (2026-09-30) PASS NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994192 F F NA Agree 60.05 0.00 0.00 0.49 0.32 0.85 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.895445 0.054135 0.034711 0.015709 95.06 3.02 1.78 90 LP2 (2026-09-30) PASS NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994193 F F NA Agree 54.19 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.898867 0.052578 0.032102 0.016453 95.14 3.16 1.57 91 LP2 (2026-09-30) PASS NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994194 F F NA Agree 49.62 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.901964 0.05013 0.032997 0.014909 95.04 2.96 1.89 88 LP2 (2026-09-30) PASS NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994195 F F NA Agree 48.07 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.895566 0.050883 0.028829 0.024722 93.78 3.92 2.17 98 LP2 (2026-09-30) PASS NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994196 F F NA Agree 53.23 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.892011 0.057243 0.031813 0.018933 94.65 3.21 2.02 79 LP2 (2026-09-30) PASS NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994197 M M NA Agree 0.00 1.80 0.00 20.04 3.86 49.23 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.900979 0.051317 0.033483 0.014221 89.27 3.70 6.88 111 LP2 (2026-09-30) PASS NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994198 F F NA Agree 49.96 0.00 0.00 0.00 0.12 0.39 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.896915 0.050785 0.033682 0.018618 94.69 3.33 1.85 81 LP2 (2026-09-30) PASS NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994199 F F NA Agree 49.28 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.900724 0.050403 0.030845 0.018028 94.79 3.34 1.72 75 LP2 (2026-09-30) PASS NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994200 M M NA Agree 0.04 4.58 0.00 28.16 13.10 55.48 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.883814 0.054201 0.032075 0.02991 93.59 4.49 1.78 76 LP2 (2026-09-30) PASS NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994201 F F NA Agree 37.89 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.910031 0.046254 0.030241 0.013474 95.40 2.80 1.65 72 LP2 (2026-09-30) PASS NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994202 F F NA Agree 57.53 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.879123 0.057742 0.030888 0.032247 93.63 4.66 1.58 80 LP2 (2026-09-30) PASS NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994203 F M NA Conflict 0.01 4.02 0.00 26.38 10.10 48.30 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.897193 0.05355 0.029505 0.019751 94.62 3.38 1.85 66 LP2 (2026-09-30) PASS NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994204 F F NA Agree 51.78 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.899395 0.050259 0.032474 0.017872 94.75 3.37 1.75 76 LP2 (2026-09-30) PASS NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994205 F F NA Agree 49.48 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.89493 0.048797 0.031019 0.025253 89.96 3.91 6.00 97 LP2 (2026-09-30) PASS NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM8994206 F F NA Agree 54.88 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.893811 0.053539 0.032392 0.020258 94.65 3.42 1.81 88 LP2 (2026-09-30) PASS NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does