Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8943423	M	M	NA	Agree	0.46	7.10	0.00	16.00	7.45	24.31	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.529759	0.37954	0.08014	0.010562	90.19	5.47	3.87	2700	LP2 (2026-09-30)	PASS	NonPolyA_perM 2700 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943424	M	M	NA	Agree	0.24	4.75	0.00	12.87	5.48	17.63	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.59745	0.30792	0.082739	0.011891	86.72	5.98	6.81	4007	LP2 (2026-09-30)	PASS	NonPolyA_perM 4007 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943425	M	M	NA	Agree	0.36	7.36	0.00	16.13	6.63	20.40	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.504601	0.407495	0.079	0.008903	88.82	5.13	5.54	3708	LP2 (2026-09-30)	PASS	NonPolyA_perM 3708 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943426	M	M	NA	Agree	0.26	6.44	0.00	14.85	6.11	18.82	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.515546	0.372443	0.099971	0.012039	85.50	5.84	8.20	3312	LP2 (2026-09-30)	PASS	NonPolyA_perM 3312 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943427	M	M	NA	Agree	0.21	5.66	0.00	14.29	5.48	19.77	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.610121	0.288842	0.092691	0.008346	87.38	6.44	5.69	4303	LP2 (2026-09-30)	PASS	NonPolyA_perM 4303 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943428	M	M	NA	Agree	0.19	6.54	0.00	15.63	6.34	19.48	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.539465	0.358665	0.084229	0.017641	80.14	6.11	13.31	3809	LP2 (2026-09-30)	PASS	NonPolyA_perM 3809 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943429	M	M	NA	Agree	0.22	4.32	0.00	9.90	4.72	16.25	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.556254	0.327003	0.096217	0.020526	78.44	7.04	14.12	4490	LP2 (2026-09-30)	PASS	NonPolyA_perM 4490 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943430	M	M	NA	Agree	0.22	4.09	0.00	10.70	4.44	13.98	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.534692	0.310197	0.09983	0.055281	78.58	11.44	9.52	6027	LP2 (2026-09-30)	PASS	NonPolyA_perM 6027 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8943431	M	M	NA	Agree	0.24	4.34	0.00	11.78	4.28	14.11	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.573938	0.308425	0.094023	0.023614	85.50	8.36	5.70	6394	LP2 (2026-09-30)	PASS	NonPolyA_perM 6394 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not