Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8895836	M	M	NA	Agree	0.04	7.54	0.00	29.70	11.62	69.58	NONE	POLYA	0.882753	0.056098	0.032765	0.028384	91.74	4.39	3.67	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895837	M	M	NA	Agree	0.02	4.95	0.00	17.88	7.57	56.28	NONE	POLYA	0.894978	0.026175	0.029287	0.04956	90.47	6.32	2.97	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895838	M	M	NA	Agree	0.01	6.67	0.00	25.03	8.35	61.98	NONE	POLYA	0.886808	0.046593	0.030971	0.035628	91.20	5.02	3.60	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895839	M	M	NA	Agree	0.01	7.43	0.00	25.18	9.34	65.27	NONE	POLYA	0.879226	0.042017	0.031513	0.047244	90.23	5.98	3.59	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895840	M	M	NA	Agree	0.02	5.85	0.00	22.87	8.55	63.80	NONE	POLYA	0.847029	0.034475	0.031104	0.087393	86.58	9.49	3.68	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895841	M	M	NA	Agree	0.02	6.92	0.00	25.76	9.30	71.83	NONE	POLYA	0.873841	0.045802	0.032755	0.047602	89.69	6.19	3.86	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895842	M	M	NA	Agree	0.00	6.43	0.00	25.36	8.78	70.65	NONE	POLYA	0.87435	0.030554	0.030801	0.064295	88.78	7.37	3.61	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895843	M	M	NA	Agree	0.02	7.50	0.00	25.00	8.01	70.91	NONE	POLYA	0.875201	0.058288	0.035499	0.031012	91.91	4.62	3.20	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895844	M	M	NA	Agree	0.03	6.07	0.00	23.97	8.42	70.36	NONE	POLYA	0.862062	0.049967	0.034933	0.053038	89.41	6.69	3.57	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895845	M	M	NA	Agree	0.03	6.46	0.00	22.51	9.07	57.86	NONE	POLYA	0.893153	0.031466	0.027903	0.047478	90.39	5.96	3.50	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895846	M	M	NA	Agree	0.02	3.68	0.00	25.54	4.59	44.90	NONE	POLYA	0.880253	0.029303	0.020779	0.069665	87.84	8.00	4.04	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895847	M	M	NA	Agree	0.00	4.70	0.00	26.57	5.55	48.83	NONE	POLYA	0.883774	0.029007	0.018446	0.068773	88.22	7.83	3.85	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895848	M	M	NA	Agree	0.00	5.03	0.00	28.39	5.01	47.29	NONE	POLYA	0.900794	0.028543	0.018786	0.051877	89.96	6.31	3.61	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895849	M	M	NA	Agree	0.00	4.12	0.00	26.60	5.42	47.25	NONE	POLYA	0.897428	0.020625	0.021325	0.060623	89.20	6.78	3.90	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895850	M	M	NA	Agree	0.01	3.89	0.00	25.98	5.27	45.33	NONE	POLYA	0.907268	0.026407	0.02046	0.045864	90.46	5.64	3.78	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895851	M	M	NA	Agree	0.00	4.71	0.00	26.97	4.96	49.23	NONE	POLYA	0.897452	0.023259	0.021046	0.058243	88.33	6.73	4.81	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895852	M	M	NA	Agree	0.00	3.50	0.00	22.20	4.80	39.45	NONE	POLYA	0.877877	0.024719	0.018171	0.079233	87.31	8.50	4.07	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895853	M	M	NA	Agree	0.00	4.22	0.00	23.04	4.72	39.91	NONE	POLYA	0.845472	0.022827	0.018373	0.113329	83.67	11.70	4.50	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895854	M	M	NA	Agree	0.01	3.67	0.00	20.54	3.92	39.93	NONE	POLYA	0.85261	0.023076	0.01985	0.104464	84.49	10.80	4.58	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8895855	M	M	NA	Agree	0.01	3.19	0.00	24.10	5.12	37.84	NONE	POLYA	0.873598	0.028304	0.019207	0.078891	87.38	8.47	4.04	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does