Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8887871	M	M	NA	Agree	0.00	5.22	0.00	22.29	5.46	32.58	NONE	POLYA	0.94484	0.025426	0.027153	0.002581	92.21	1.60	6.06	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887872	M	M	NA	Agree	0.00	4.68	0.00	23.12	3.91	38.54	NONE	POLYA	0.945169	0.02551	0.026515	0.002806	92.66	1.63	5.58	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887873	M	M	NA	Agree	0.02	4.39	0.00	21.82	5.40	39.16	NONE	POLYA	0.944481	0.025475	0.026701	0.003343	92.54	1.66	5.66	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887874	M	M	NA	Agree	0.00	0.75	0.00	4.86	3.07	9.78	NONE	POLYA	0.949491	0.021662	0.02513	0.003717	93.07	1.78	5.01	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887875	M	M	NA	Agree	0.00	1.01	0.00	4.45	2.20	13.63	NONE	POLYA	0.948885	0.02112	0.024824	0.005171	92.95	1.93	4.98	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887876	M	M	NA	Agree	0.00	0.80	0.00	4.54	3.86	11.75	NONE	POLYA	0.949919	0.021247	0.024803	0.004031	93.50	1.81	4.55	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887877	M	M	NA	Agree	0.03	3.32	0.00	20.16	5.76	40.54	NONE	POLYA	0.947471	0.023326	0.025003	0.0042	92.07	1.70	6.07	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887878	M	M	NA	Agree	0.04	4.15	0.00	17.57	5.02	36.64	NONE	POLYA	0.946311	0.02363	0.025255	0.004804	91.77	1.75	6.32	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8887879	M	M	NA	Agree	0.00	2.97	0.00	20.78	5.01	33.90	NONE	POLYA	0.947179	0.023554	0.025386	0.003882	92.89	1.71	5.23	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does