Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8873773	M	M	NA	Agree	0.02	3.43	0.00	42.68	8.56	122.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.941457	0.025973	0.030597	0.001973	82.36	1.81	15.59	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873774	M	M	NA	Agree	0.00	3.55	0.00	32.17	8.08	97.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936006	0.022897	0.029745	0.011352	80.25	2.69	16.82	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873775	M	M	NA	Agree	0.02	3.66	0.00	35.66	10.87	91.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.92692	0.029618	0.035376	0.008086	80.78	2.36	16.63	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873776	M	M	NA	Agree	0.00	1.09	0.00	21.35	7.58	75.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915715	0.042232	0.03714	0.004913	82.44	2.19	15.08	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873777	M	M	NA	Agree	0.01	1.19	0.00	21.09	8.91	89.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930531	0.032447	0.034155	0.002867	83.12	1.81	14.82	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873778	M	M	NA	Agree	0.00	1.82	0.00	27.18	8.58	100.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.926336	0.034273	0.035428	0.003963	83.69	1.81	14.28	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873779	M	M	NA	Agree	0.01	0.63	0.00	20.12	8.06	80.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924709	0.038389	0.035127	0.001775	83.00	1.64	15.09	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873780	M	M	NA	Agree	0.01	1.40	0.00	25.53	7.27	82.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939298	0.032041	0.027459	0.001202	81.46	1.81	16.43	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873781	M	M	NA	Agree	0.01	1.55	0.00	30.70	8.07	71.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935431	0.037073	0.025998	0.001498	83.39	2.03	14.16	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873782	M	M	NA	Agree	0.00	1.32	0.00	23.20	7.22	81.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935486	0.029821	0.03246	0.002234	82.74	1.93	15.11	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873783	M	M	NA	Agree	0.00	1.58	0.00	19.58	5.49	71.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937792	0.032787	0.026596	0.002826	79.98	1.82	17.81	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873784	M	M	NA	Agree	0.01	1.44	0.00	32.03	7.40	69.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.931293	0.039502	0.025914	0.003292	80.25	2.07	17.34	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873785	M	M	NA	Agree	0.01	0.96	0.00	32.00	7.81	71.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937422	0.033404	0.027592	0.001583	81.33	1.63	16.71	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873786	M	M	NA	Agree	0.01	1.93	0.00	26.03	7.12	79.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930753	0.039558	0.02642	0.003268	82.04	1.97	15.65	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873787	M	M	NA	Agree	0.01	2.21	0.00	33.49	6.47	74.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930712	0.04014	0.026308	0.00284	81.95	1.87	15.73	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873788	M	M	NA	Agree	0.02	2.25	0.00	29.61	5.46	89.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933325	0.040253	0.024868	0.001555	81.96	1.68	15.97	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873789	M	M	NA	Agree	0.01	2.29	0.00	34.75	7.49	77.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933675	0.038124	0.025939	0.002262	82.68	1.63	15.37	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873790	M	M	NA	Agree	0.00	2.37	0.00	30.85	6.33	76.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.934728	0.035118	0.025439	0.004715	82.68	2.06	14.86	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873791	M	M	NA	Agree	0.02	2.69	0.00	37.43	8.57	92.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.931775	0.038144	0.027954	0.002127	82.32	1.71	15.73	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8873792	M	M	NA	Agree	0.06	2.74	0.00	42.43	9.37	103.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923582	0.041921	0.030766	0.003731	84.06	2.05	13.66	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does