Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8846148	M	M	NA	Agree	0.06	3.65	0.00	19.11	8.03	71.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91358	0.03944	0.034862	0.012118	87.33	3.85	8.39	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8846149	M	M	NA	Agree	0.11	3.49	0.00	19.01	14.60	75.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.929351	0.032749	0.032581	0.005319	89.76	3.13	6.60	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8846150	M	M	NA	Agree	0.15	5.16	0.00	29.91	18.62	66.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873549	0.086974	0.034985	0.004493	92.10	3.50	3.98	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8846151	M	M	NA	Agree	0.09	2.71	0.00	14.67	11.93	48.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912771	0.033035	0.02968	0.024513	91.20	4.84	3.46	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8846152	M	M	NA	Agree	0.03	2.85	0.00	9.50	9.65	50.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.949235	0.018028	0.027045	0.005692	92.94	4.00	2.66	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8846153	M	M	NA	Agree	0.03	1.87	0.03	7.65	8.62	43.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940269	0.017104	0.027734	0.014892	90.95	4.67	3.95	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does