Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8806205	M	M	NA	Agree	0.00	6.59	0.00	45.35	12.49	75.87	NONE	POLYA	0.836439	0.099527	0.038096	0.025938	85.45	3.85	10.56	156	LP2 (2026-09-30)	PASS	NonPolyA_perM 156 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806206	M	M	NA	Agree	0.03	6.78	0.00	44.46	9.51	57.17	NONE	POLYA	0.594994	0.122506	0.033398	0.249102	67.50	23.12	9.21	203	LP2 (2026-09-30)	PASS	NonPolyA_perM 203 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806207	M	M	NA	Agree	0.00	5.10	0.00	39.00	11.27	51.67	NONE	POLYA	0.645715	0.088105	0.036049	0.230132	68.83	21.19	9.80	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806208	M	M	NA	Agree	0.00	5.31	0.00	36.00	11.06	68.04	NONE	POLYA	0.757614	0.105905	0.036978	0.099503	80.32	10.82	8.68	162	LP2 (2026-09-30)	PASS	NonPolyA_perM 162 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806209	M	M	NA	Agree	0.00	5.73	0.00	40.43	9.78	56.77	NONE	POLYA	0.588458	0.13068	0.035728	0.245134	68.73	22.57	8.52	210	LP2 (2026-09-30)	PASS	NonPolyA_perM 210 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806210	M	M	NA	Agree	0.00	5.12	0.00	49.18	8.85	42.42	NONE	POLYA	0.594145	0.079015	0.034467	0.292374	64.22	26.77	8.82	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806211	M	M	NA	Agree	0.00	6.35	0.00	33.43	14.53	55.41	NONE	POLYA	0.641884	0.123832	0.034827	0.199458	72.03	18.82	9.01	188	LP2 (2026-09-30)	PASS	NonPolyA_perM 188 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806212	M	M	NA	Agree	0.01	3.89	0.00	26.83	10.47	55.24	NONE	POLYA	0.834639	0.078087	0.029084	0.058191	84.95	6.50	8.42	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806213	M	M	NA	Agree	0.01	5.47	0.00	21.68	13.84	57.46	NONE	POLYA	0.854515	0.072842	0.037966	0.034677	84.52	4.95	10.27	154	LP2 (2026-09-30)	PASS	NonPolyA_perM 154 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806214	M	M	NA	Agree	0.00	6.00	0.00	31.06	10.69	61.42	NONE	POLYA	0.817853	0.080648	0.02696	0.074539	82.58	7.96	9.33	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806215	M	M	NA	Agree	0.00	5.93	0.00	33.62	12.25	68.22	NONE	POLYA	0.820906	0.09	0.031778	0.057317	84.44	6.27	9.16	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8806216	M	M	NA	Agree	0.00	4.76	0.00	29.88	10.84	29.60	NONE	POLYA	0.894074	0.050325	0.019975	0.035626	86.25	4.43	9.21	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does