Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8802868	F	F	NA	Agree	83.60	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.9268	0.029628	0.040709	0.002862	84.91	1.98	12.91	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802869	F	F	NA	Agree	87.70	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.931794	0.027849	0.035258	0.0051	86.65	2.01	11.15	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802870	F	F	NA	Agree	50.54	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.93216	0.022996	0.029805	0.015039	90.38	3.02	6.44	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802871	F	F	NA	Agree	66.78	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.933106	0.030364	0.031494	0.005037	91.63	2.25	5.94	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802872	F	F	NA	Agree	84.14	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.931069	0.029283	0.036244	0.003404	92.20	2.19	5.44	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802873	F	F	NA	Agree	56.11	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.930295	0.026327	0.031756	0.011622	89.93	2.73	7.20	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802874	F	F	NA	Agree	79.64	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.934075	0.027721	0.0343	0.003904	91.96	2.18	5.72	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802875	F	F	NA	Agree	45.95	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.940238	0.021522	0.031485	0.006754	90.73	2.33	6.78	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802876	F	F	NA	Agree	37.04	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.925744	0.023169	0.037217	0.01387	87.41	2.99	9.38	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802877	F	F	NA	Agree	154.58	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.916115	0.041106	0.038104	0.004675	90.09	2.27	7.47	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802878	F	F	NA	Agree	64.46	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.926629	0.028559	0.033287	0.011525	90.79	2.99	6.05	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802879	F	F	NA	Agree	90.21	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.931912	0.027712	0.036206	0.00417	87.87	2.20	9.77	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802880	F	F	NA	Agree	42.34	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.906904	0.027366	0.03858	0.02715	89.96	4.36	5.46	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802881	F	F	NA	Agree	71.59	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.899143	0.030996	0.033714	0.036147	89.06	5.05	5.66	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8802882	F	F	NA	Agree	50.21	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.936463	0.022073	0.033764	0.0077	88.67	2.43	8.75	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does