Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8760293	M	M	NA	Agree	0.02	1.44	0.00	14.83	1.49	24.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.932082	0.019923	0.018256	0.029739	91.55	5.04	3.26	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8760294	M	M	NA	Agree	0.00	4.44	0.00	21.20	7.37	49.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.928878	0.036592	0.022841	0.011689	94.46	2.97	2.41	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8760295	M	M	NA	Agree	0.00	4.17	0.00	19.43	5.42	44.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91967	0.039026	0.024477	0.016827	92.49	3.77	3.52	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8760296	M	M	NA	Agree	0.00	4.52	0.00	22.50	5.13	44.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912926	0.047501	0.027533	0.012039	94.49	3.26	2.08	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8760297	M	M	NA	Agree	0.00	2.51	0.00	16.84	5.80	39.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918016	0.042821	0.025433	0.01373	93.34	3.55	2.90	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8760298	M	M	NA	Agree	0.00	3.35	0.00	23.36	5.48	46.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.927054	0.027907	0.020291	0.024749	92.75	4.45	2.65	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does