Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8699458	M	M	NA	Agree	0.00	3.32	0.00	31.02	7.02	58.64	NONE	POLYA	0.934979	0.028172	0.034573	0.002276	94.84	1.82	3.13	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8699459	M	M	NA	Agree	0.03	4.31	0.00	27.14	10.98	64.30	NONE	POLYA	0.93603	0.027446	0.034288	0.002236	94.75	1.67	3.44	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8699460	M	M	NA	Agree	0.01	6.17	0.00	27.14	11.66	60.57	NONE	POLYA	0.929503	0.03279	0.035837	0.001869	94.94	1.63	3.30	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8699461	M	M	NA	Agree	0.01	6.72	0.00	30.99	12.49	75.80	NONE	POLYA	0.932738	0.032201	0.033569	0.001492	94.75	1.56	3.56	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8699462	M	M	NA	Agree	0.01	3.91	0.00	19.32	10.46	50.06	NONE	POLYA	0.932664	0.030267	0.034832	0.002237	94.45	1.59	3.84	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8699463	M	M	NA	Agree	0.00	4.79	0.00	28.99	10.08	79.41	NONE	POLYA	0.933275	0.030057	0.03403	0.002639	94.02	1.75	4.11	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does