Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8683562	M	M	NA	Agree	0.00	4.09	0.00	26.77	14.64	52.91	NONE	POLYA	0.923558	0.023231	0.04117	0.01204	83.15	2.22	14.51	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683563	M	M	NA	Agree	0.00	4.08	0.00	24.54	13.48	56.59	NONE	POLYA	0.925487	0.020094	0.039574	0.014845	83.99	2.53	13.35	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683564	M	M	NA	Agree	0.00	5.89	0.00	22.62	13.23	56.63	NONE	POLYA	0.926805	0.022902	0.040812	0.009482	84.12	2.09	13.67	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683565	M	M	NA	Agree	0.00	5.18	0.00	26.28	15.08	56.35	NONE	POLYA	0.927849	0.024566	0.041885	0.0057	81.52	1.62	16.74	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683566	M	M	NA	Agree	0.00	5.17	0.00	29.03	15.42	58.88	NONE	POLYA	0.924475	0.025854	0.041694	0.007976	82.26	1.79	15.83	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683567	M	M	NA	Agree	0.00	4.63	0.00	21.54	12.74	58.62	NONE	POLYA	0.925983	0.021498	0.040686	0.011833	83.87	2.25	13.77	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683568	M	M	NA	Agree	0.00	5.71	0.00	18.81	13.32	55.41	NONE	POLYA	0.931915	0.018492	0.039196	0.010397	82.13	2.09	15.67	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683569	M	M	NA	Agree	0.00	4.55	0.00	24.61	12.43	57.33	NONE	POLYA	0.922981	0.023843	0.04032	0.012856	84.04	2.37	13.47	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683570	M	M	NA	Agree	0.00	7.02	0.00	27.04	13.32	56.44	NONE	POLYA	0.922301	0.02751	0.040783	0.009406	84.85	2.00	13.02	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683571	M	M	NA	Agree	0.00	4.78	0.00	24.97	12.97	51.90	NONE	POLYA	0.927441	0.024076	0.040658	0.007825	89.36	1.95	8.55	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683572	M	M	NA	Agree	0.00	4.80	0.00	20.00	11.59	52.63	NONE	POLYA	0.925561	0.019047	0.040645	0.014747	81.05	2.40	16.43	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683573	M	M	NA	Agree	0.00	5.02	0.00	25.00	14.44	57.52	NONE	POLYA	0.925156	0.021967	0.042289	0.010588	82.81	2.09	14.98	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683574	M	M	NA	Agree	0.00	4.64	0.00	23.13	12.50	48.00	NONE	POLYA	0.925087	0.020608	0.039756	0.014548	84.15	2.56	13.17	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683575	M	M	NA	Agree	0.00	4.50	0.00	26.87	12.50	56.95	NONE	POLYA	0.920656	0.025742	0.040517	0.013084	82.79	2.32	14.75	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8683576	M	M	NA	Agree	0.00	6.05	0.00	27.21	10.56	63.26	NONE	POLYA	0.91029	0.034796	0.04114	0.013774	83.57	2.45	13.84	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does