Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8658278	M	M	NA	Agree	0.00	5.05	0.00	21.03	8.72	45.52	NONE	POLYA	0.903134	0.051456	0.043996	0.001415	93.91	1.65	4.30	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658279	M	M	NA	Agree	0.00	3.73	0.00	19.66	10.55	38.42	NONE	POLYA	0.897715	0.060233	0.041474	0.000578	94.51	1.49	3.85	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658280	M	M	NA	Agree	0.00	4.03	0.00	20.54	11.32	35.82	NONE	POLYA	0.901555	0.054018	0.043308	0.001119	94.89	1.52	3.46	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658281	M	M	NA	Agree	0.00	3.14	0.00	17.99	8.96	40.06	NONE	POLYA	0.9086	0.048181	0.042573	0.000646	94.49	1.50	3.87	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658282	M	M	NA	Agree	0.01	5.01	0.00	21.45	9.06	55.69	NONE	POLYA	0.904138	0.051249	0.043428	0.001185	93.80	1.59	4.47	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658283	M	M	NA	Agree	0.00	5.08	0.00	18.80	8.14	39.34	NONE	POLYA	0.907015	0.048222	0.043404	0.001359	93.90	1.59	4.38	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658284	M	M	NA	Agree	0.00	2.74	0.00	17.12	8.17	37.84	NONE	POLYA	0.924776	0.033326	0.040897	0.001001	94.35	1.50	4.02	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658285	M	M	NA	Agree	0.00	4.38	0.00	17.54	9.12	42.02	NONE	POLYA	0.911576	0.045241	0.042313	0.00087	94.30	1.48	4.08	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658286	M	M	NA	Agree	0.00	3.24	0.00	16.67	8.94	37.43	NONE	POLYA	0.909776	0.047164	0.042323	0.000738	94.56	1.46	3.83	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658287	M	M	NA	Agree	0.00	3.74	0.00	17.08	8.24	40.06	NONE	POLYA	0.900809	0.055215	0.043154	0.000822	94.25	1.60	4.01	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658288	M	M	NA	Agree	0.00	4.45	0.00	18.36	9.82	35.66	NONE	POLYA	0.905177	0.051305	0.042863	0.000655	94.17	1.49	4.22	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8658289	M	M	NA	Agree	0.01	1.97	0.00	12.55	6.84	27.22	NONE	POLYA	0.908919	0.050652	0.03971	0.000719	94.93	1.42	3.52	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does