Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8654521	M	M	NA	Agree	0.42	23.43	0.00	34.01	16.39	21.93	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.604799	0.3478	0.046596	0.000805	77.28	1.84	20.23	2084	LP2 (2026-09-30)	PASS	NonPolyA_perM 2084 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654522	M	M	NA	Agree	0.10	20.00	0.00	44.40	13.56	33.76	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.581218	0.370325	0.046856	0.0016	81.15	1.92	16.24	2089	LP2 (2026-09-30)	PASS	NonPolyA_perM 2089 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654523	M	M	NA	Agree	0.06	14.68	0.00	40.34	9.60	35.77	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.588475	0.363765	0.04704	0.000719	81.79	1.91	15.59	1963	LP2 (2026-09-30)	PASS	NonPolyA_perM 1963 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654524	M	M	NA	Agree	0.29	19.74	0.00	43.80	16.26	33.49	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.555913	0.39315	0.048159	0.002777	81.10	1.97	16.28	1640	LP2 (2026-09-30)	PASS	NonPolyA_perM 1640 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654525	M	M	NA	Agree	0.09	14.35	0.00	35.02	16.73	28.89	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.598289	0.352619	0.047678	0.001414	79.54	2.05	17.71	2213	LP2 (2026-09-30)	PASS	NonPolyA_perM 2213 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654526	M	M	NA	Agree	0.28	18.89	0.00	52.56	12.80	29.20	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.570237	0.380895	0.047264	0.001603	78.07	1.79	19.48	2101	LP2 (2026-09-30)	PASS	NonPolyA_perM 2101 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654527	M	M	NA	Agree	0.20	26.97	0.00	50.96	18.98	47.05	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.534775	0.414619	0.049641	0.000965	82.52	1.76	15.03	1513	LP2 (2026-09-30)	PASS	NonPolyA_perM 1513 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654528	M	M	NA	Agree	0.20	14.30	0.00	38.89	13.71	23.94	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.545025	0.402198	0.051659	0.001118	77.00	2.04	20.12	3503	LP2 (2026-09-30)	PASS	NonPolyA_perM 3503 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654529	M	M	NA	Agree	0.11	16.87	0.00	35.90	12.37	35.58	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.580329	0.369243	0.049045	0.001383	82.78	2.00	14.55	2308	LP2 (2026-09-30)	PASS	NonPolyA_perM 2308 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654530	M	M	NA	Agree	0.13	20.77	0.00	54.82	17.11	37.41	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.575196	0.379435	0.044696	0.000673	81.67	1.73	16.02	1404	LP2 (2026-09-30)	PASS	NonPolyA_perM 1404 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654531	M	M	NA	Agree	0.25	19.59	0.00	42.08	17.30	27.35	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.56105	0.389312	0.048452	0.001186	80.26	1.93	17.10	2485	LP2 (2026-09-30)	PASS	NonPolyA_perM 2485 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8654532	M	M	NA	Agree	0.69	20.90	0.00	33.63	16.66	42.34	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.574934	0.376492	0.047432	0.001142	82.70	1.91	14.78	1688	LP2 (2026-09-30)	PASS	NonPolyA_perM 1688 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not