Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8649266	not_specified	M	NA	Conflict	0.02	3.10	0.00	20.90	7.75	33.26	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.923655	0.046386	0.022683	0.007276	78.50	2.39	18.76	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649267	not_specified	M	NA	Conflict	0.00	3.88	0.00	23.53	7.95	36.25	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.9226	0.046211	0.024735	0.006454	58.51	1.88	39.35	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649268	not_specified	M	NA	Conflict	0.00	2.57	0.00	17.88	6.79	39.09	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.920808	0.049281	0.02261	0.007301	81.37	2.39	15.98	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649269	not_specified	F	NA	Conflict	15.37	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.940971	0.031378	0.022372	0.005278	76.55	2.42	20.66	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649270	not_specified	M	NA	Conflict	0.33	5.17	0.00	26.14	7.49	38.26	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.921494	0.045875	0.024584	0.008046	77.31	2.19	20.25	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649271	not_specified	M	NA	Conflict	0.04	2.69	0.00	23.43	8.65	38.96	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.919704	0.042667	0.022076	0.015553	73.02	2.90	23.83	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649272	not_specified	M	NA	Conflict	0.01	3.39	0.00	18.56	7.04	31.19	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.929784	0.040385	0.022713	0.007118	75.39	2.28	22.03	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649273	not_specified	F	NA	Conflict	89.27	0.00	0.00	0.00	0.00	0.00	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.910884	0.054626	0.029465	0.005025	74.09	2.85	21.77	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649274	not_specified	M	NA	Conflict	2.67	3.84	0.00	14.84	6.01	36.77	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.927671	0.037977	0.031936	0.002416	86.59	2.81	8.81	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649275	not_specified	M	NA	Conflict	0.31	2.71	0.00	12.83	5.19	38.97	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.938598	0.027697	0.031186	0.002518	85.33	2.86	10.39	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649276	not_specified	M	NA	Conflict	0.00	5.81	0.00	29.66	10.37	39.28	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.789974	0.081753	0.04027	0.088003	68.47	8.87	16.84	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649277	not_specified	M	NA	Conflict	0.04	2.24	0.00	12.64	4.15	39.41	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.947183	0.02308	0.025582	0.004155	75.82	2.70	20.37	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does