Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8647208	M	M	NA	Agree	0.00	2.64	0.00	18.94	6.72	24.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.92433	0.052881	0.019389	0.003401	95.35	2.21	2.02	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647209	M	M	NA	Agree	0.01	2.90	0.00	22.31	6.51	28.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.919332	0.056192	0.020194	0.004281	94.91	2.54	2.09	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647210	M	M	NA	Agree	0.00	2.79	0.00	19.60	7.11	25.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921343	0.05497	0.019879	0.003808	95.07	2.34	2.13	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647211	M	M	NA	Agree	0.02	2.49	0.00	18.33	6.09	26.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923898	0.053499	0.018875	0.003729	94.63	2.31	2.64	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647212	M	M	NA	Agree	0.02	3.58	0.00	24.93	7.37	28.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.9209	0.056809	0.019451	0.00284	94.65	2.23	2.65	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647213	M	M	NA	Agree	0.01	3.42	0.00	24.38	6.78	29.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920368	0.056036	0.020476	0.00312	94.95	2.20	2.38	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647214	M	M	NA	Agree	0.00	1.65	0.00	19.77	7.30	27.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.914454	0.061193	0.020802	0.003551	94.22	2.66	2.69	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647215	M	M	NA	Agree	0.02	1.85	0.00	17.55	5.33	26.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930025	0.047992	0.018988	0.002995	94.45	2.42	2.76	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647216	M	M	NA	Agree	0.01	1.64	0.00	18.41	5.96	28.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923946	0.052388	0.019888	0.003778	94.30	2.47	2.80	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647217	M	M	NA	Agree	0.02	2.24	0.00	17.70	5.73	28.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921818	0.055288	0.019913	0.00298	94.41	2.48	2.73	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647218	M	M	NA	Agree	0.01	1.93	0.00	17.29	5.99	24.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921716	0.055458	0.019911	0.002915	95.43	2.38	1.77	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647219	M	M	NA	Agree	0.00	2.65	0.00	19.99	6.21	29.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913768	0.062214	0.020504	0.003514	95.26	2.50	1.82	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647220	M	M	NA	Agree	0.01	2.79	0.00	22.42	7.16	27.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91313	0.063068	0.019968	0.003835	95.60	2.48	1.49	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647221	M	M	NA	Agree	0.01	2.47	0.00	23.64	7.52	31.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921335	0.054159	0.020078	0.004428	95.22	2.61	1.76	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647222	M	M	NA	Agree	0.01	2.85	0.00	23.16	7.12	27.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921104	0.053794	0.02051	0.004592	95.52	2.42	1.60	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8647223	M	M	NA	Agree	0.00	2.05	0.00	20.46	5.57	27.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.928947	0.048559	0.018192	0.004302	95.17	2.37	2.02	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does