Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8601783	F	F	NA	Agree	132.94	0.06	0.00	0.23	0.03	0.66	NONE	POLYA	0.835515	0.113729	0.046299	0.004457	92.29	1.68	5.84	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601784	F	F	NA	Agree	126.63	0.06	0.00	0.22	0.00	0.35	NONE	POLYA	0.833261	0.114556	0.045038	0.007145	92.18	1.99	5.65	141	LP2 (2026-09-30)	PASS	NonPolyA_perM 141 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601785	F	F	NA	Agree	132.09	0.03	0.00	0.12	0.11	0.08	NONE	POLYA	0.842689	0.107149	0.0452	0.004962	92.54	1.77	5.50	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601786	F	F	NA	Agree	155.07	0.03	0.00	0.10	0.04	0.38	NONE	POLYA	0.825493	0.123325	0.046939	0.004242	92.60	1.70	5.51	139	LP2 (2026-09-30)	PASS	NonPolyA_perM 139 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601787	F	F	NA	Agree	113.91	0.06	0.00	0.18	0.07	0.44	NONE	POLYA	0.846715	0.101169	0.045755	0.006361	92.49	1.83	5.49	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601788	F	F	NA	Agree	134.75	0.06	0.00	0.17	0.07	0.25	NONE	POLYA	0.840694	0.109778	0.046096	0.003432	92.80	1.68	5.32	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601789	F	F	NA	Agree	87.80	0.00	0.00	0.11	0.02	0.15	NONE	POLYA	0.855937	0.090506	0.046703	0.006854	92.63	1.94	5.24	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601790	F	F	NA	Agree	107.95	0.00	0.00	0.09	0.00	0.26	NONE	POLYA	0.853611	0.095472	0.045264	0.005653	92.82	1.81	5.20	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601791	F	F	NA	Agree	92.41	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.844284	0.10799	0.042832	0.004894	87.37	1.81	10.60	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601792	F	F	NA	Agree	117.80	0.08	0.00	0.27	0.07	0.31	NONE	POLYA	0.850589	0.100553	0.042547	0.006311	92.53	1.99	5.29	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601793	F	F	NA	Agree	105.42	0.00	0.00	0.11	0.04	0.16	NONE	POLYA	0.840681	0.108578	0.046295	0.004446	92.86	1.73	5.21	135	LP2 (2026-09-30)	PASS	NonPolyA_perM 135 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601794	F	F	NA	Agree	92.59	0.05	0.00	0.13	0.07	0.15	NONE	POLYA	0.856342	0.092118	0.044355	0.007186	92.82	1.98	5.02	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601795	F	F	NA	Agree	102.33	0.04	0.00	0.11	0.19	0.30	NONE	POLYA	0.848301	0.102927	0.044886	0.003885	92.75	1.70	5.36	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601796	F	F	NA	Agree	127.27	0.00	0.00	0.06	0.00	0.08	NONE	POLYA	0.835021	0.113388	0.046431	0.00516	92.65	1.77	5.38	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601797	F	F	NA	Agree	122.14	0.02	0.00	0.17	0.04	0.28	NONE	POLYA	0.838706	0.107102	0.045355	0.008838	92.15	2.10	5.55	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601798	F	F	NA	Agree	116.75	0.06	0.00	0.17	0.04	0.32	NONE	POLYA	0.841949	0.108897	0.046194	0.002959	92.95	1.56	5.30	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601799	F	F	NA	Agree	123.49	0.00	0.00	0.08	0.03	0.32	NONE	POLYA	0.840913	0.109344	0.044798	0.004945	92.52	1.76	5.53	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601800	F	F	NA	Agree	108.91	0.00	0.00	0.27	0.04	0.20	NONE	POLYA	0.841538	0.10822	0.044925	0.005317	92.50	1.78	5.52	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601801	M	M	NA	Agree	0.62	5.63	0.00	26.84	11.66	61.62	NONE	POLYA	0.838365	0.11264	0.046228	0.002766	92.33	1.60	5.88	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601802	M	M	NA	Agree	0.63	6.29	0.00	30.91	9.13	67.78	NONE	POLYA	0.83032	0.119646	0.045961	0.004073	92.63	1.71	5.47	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601803	M	M	NA	Agree	0.46	5.04	0.00	25.96	13.28	68.50	NONE	POLYA	0.819727	0.128287	0.047659	0.004326	92.58	1.82	5.39	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601804	M	M	NA	Agree	0.28	5.89	0.00	27.05	10.54	61.56	NONE	POLYA	0.839689	0.112565	0.044938	0.002807	92.77	1.59	5.44	136	LP2 (2026-09-30)	PASS	NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601805	M	M	NA	Agree	0.35	5.81	0.00	29.31	11.62	56.42	NONE	POLYA	0.829567	0.120369	0.047066	0.002997	92.76	1.60	5.45	153	LP2 (2026-09-30)	PASS	NonPolyA_perM 153 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601806	M	M	NA	Agree	0.49	5.77	0.00	26.86	10.57	52.44	NONE	POLYA	0.842034	0.105024	0.046102	0.006839	92.72	2.04	5.05	151	LP2 (2026-09-30)	PASS	NonPolyA_perM 151 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601807	M	M	NA	Agree	0.66	6.06	0.00	26.48	9.77	54.93	NONE	POLYA	0.840424	0.110945	0.043825	0.004807	92.51	1.76	5.54	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601808	M	M	NA	Agree	0.18	5.80	0.00	30.22	14.28	60.93	NONE	POLYA	0.839313	0.109693	0.046308	0.004686	92.84	1.72	5.25	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601809	M	M	NA	Agree	0.35	6.14	0.00	28.80	12.41	57.82	NONE	POLYA	0.850006	0.09993	0.046287	0.003777	92.74	1.60	5.50	141	LP2 (2026-09-30)	PASS	NonPolyA_perM 141 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601810	M	M	NA	Agree	0.47	6.64	0.00	28.49	10.72	59.59	NONE	POLYA	0.836715	0.110348	0.046415	0.006522	92.52	1.89	5.39	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601811	M	M	NA	Agree	0.38	5.42	0.00	34.74	13.47	65.35	NONE	POLYA	0.844269	0.105184	0.046281	0.004266	92.74	1.73	5.34	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601812	M	M	NA	Agree	0.19	5.38	0.00	25.94	11.66	61.51	NONE	POLYA	0.839586	0.110565	0.046038	0.00381	92.64	1.73	5.44	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601813	M	M	NA	Agree	0.59	6.19	0.00	31.25	12.77	54.17	NONE	POLYA	0.82875	0.120291	0.046961	0.003998	92.61	1.69	5.49	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601814	M	M	NA	Agree	0.39	5.33	0.00	31.29	11.45	62.30	NONE	POLYA	0.847755	0.103439	0.046	0.002806	92.91	1.54	5.37	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601815	M	M	NA	Agree	0.56	5.13	0.00	29.51	9.70	53.97	NONE	POLYA	0.820265	0.120796	0.044393	0.014546	91.88	2.63	5.28	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601816	M	M	NA	Agree	0.35	7.15	0.00	26.35	12.88	59.66	NONE	POLYA	0.83103	0.119028	0.045092	0.00485	92.36	1.78	5.66	138	LP2 (2026-09-30)	PASS	NonPolyA_perM 138 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does