Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8601599	M	M	NA	Agree	0.01	3.24	0.00	15.17	6.26	31.67	NONE	POLYA	0.483752	0.135702	0.034096	0.34645	61.97	33.71	4.11	173	LP2 (2026-09-30)	PASS	NonPolyA_perM 173 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601600	M	M	NA	Agree	0.01	6.33	0.00	29.10	13.19	64.11	NONE	POLYA	0.824055	0.113442	0.047331	0.015173	91.12	2.87	5.83	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601601	M	M	NA	Agree	0.08	6.79	0.00	28.30	13.68	64.80	NONE	POLYA	0.79546	0.141502	0.047379	0.015659	91.10	2.89	5.82	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601602	M	M	NA	Agree	0.02	6.41	0.00	28.21	13.77	55.36	NONE	POLYA	0.811844	0.129121	0.046151	0.012883	91.36	2.69	5.77	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601603	M	M	NA	Agree	0.01	6.78	0.00	28.18	12.65	59.65	NONE	POLYA	0.800356	0.136921	0.047505	0.015218	91.27	2.76	5.77	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601604	M	M	NA	Agree	0.02	6.55	0.00	27.32	12.96	61.90	NONE	POLYA	0.839683	0.107059	0.046113	0.007146	91.82	2.02	5.99	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601605	M	M	NA	Agree	0.00	1.16	0.00	6.91	2.84	28.74	NONE	POLYA	0.859494	0.059257	0.04044	0.040809	88.33	5.65	5.90	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601606	M	M	NA	Agree	0.02	5.68	0.00	36.24	11.66	61.63	NONE	POLYA	0.829485	0.112592	0.047338	0.010586	92.56	2.47	4.79	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601607	M	M	NA	Agree	0.03	5.55	0.00	38.34	13.57	70.27	NONE	POLYA	0.850863	0.094545	0.048021	0.006571	92.71	2.08	5.03	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601608	M	M	NA	Agree	0.00	5.13	0.00	32.35	12.79	58.47	NONE	POLYA	0.835308	0.112918	0.046071	0.005703	92.67	2.04	5.11	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601609	M	M	NA	Agree	0.00	2.42	0.00	13.76	4.84	25.01	NONE	POLYA	0.46868	0.125425	0.032366	0.37353	58.56	37.33	3.87	214	LP2 (2026-09-30)	PASS	NonPolyA_perM 214 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601610	M	M	NA	Agree	0.01	4.91	0.00	34.11	13.54	57.07	NONE	POLYA	0.81874	0.119233	0.046037	0.015989	90.70	2.88	6.23	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601611	M	M	NA	Agree	0.03	3.12	0.00	18.90	7.48	34.17	NONE	POLYA	0.506889	0.125085	0.035949	0.332077	62.73	33.33	3.72	184	LP2 (2026-09-30)	PASS	NonPolyA_perM 184 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601612	M	M	NA	Agree	0.01	2.79	0.00	16.23	7.00	31.01	NONE	POLYA	0.478922	0.120406	0.033747	0.366925	59.59	36.60	3.60	159	LP2 (2026-09-30)	PASS	NonPolyA_perM 159 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601613	M	M	NA	Agree	0.01	2.36	0.00	13.53	6.70	30.09	NONE	POLYA	0.426833	0.124814	0.030898	0.417455	53.97	41.11	4.67	207	LP2 (2026-09-30)	PASS	NonPolyA_perM 207 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601614	M	M	NA	Agree	0.04	4.41	0.00	33.00	11.66	70.19	NONE	POLYA	0.837046	0.102497	0.049249	0.011208	91.88	2.49	5.46	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601615	M	M	NA	Agree	0.01	6.29	0.00	31.02	14.41	63.21	NONE	POLYA	0.825195	0.114518	0.046404	0.013883	91.60	2.65	5.57	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601616	M	M	NA	Agree	0.04	5.02	0.00	28.81	11.80	59.22	NONE	POLYA	0.796678	0.127396	0.047313	0.028613	90.39	4.08	5.33	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601617	M	M	NA	Agree	0.01	4.73	0.00	33.68	12.09	64.52	NONE	POLYA	0.799642	0.134109	0.04867	0.017578	91.24	3.19	5.38	136	LP2 (2026-09-30)	PASS	NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601618	M	M	NA	Agree	0.03	5.66	0.00	25.51	12.31	54.57	NONE	POLYA	0.796689	0.134985	0.048174	0.020153	90.44	3.38	5.99	138	LP2 (2026-09-30)	PASS	NonPolyA_perM 138 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601619	M	M	NA	Agree	0.02	5.06	0.00	29.93	11.32	61.89	NONE	POLYA	0.801429	0.132517	0.050175	0.015879	91.36	2.93	5.51	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601620	M	M	NA	Agree	0.05	4.75	0.00	23.60	10.11	43.27	NONE	POLYA	0.555391	0.164359	0.041813	0.238438	71.78	24.08	3.90	184	LP2 (2026-09-30)	PASS	NonPolyA_perM 184 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601621	M	M	NA	Agree	0.00	3.62	0.00	13.58	6.04	26.61	NONE	POLYA	0.428018	0.165541	0.037574	0.368868	58.96	36.69	4.07	233	LP2 (2026-09-30)	PASS	NonPolyA_perM 233 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8601622	M	M	NA	Agree	0.01	3.29	0.00	16.06	7.16	27.64	NONE	POLYA	0.461539	0.164158	0.040159	0.334143	62.30	33.40	4.03	205	LP2 (2026-09-30)	PASS	NonPolyA_perM 205 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does