Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8586707	M	M	NA	Agree	0.05	7.13	0.00	41.42	23.88	62.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868121	0.077417	0.041312	0.01315	91.37	3.21	5.19	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586708	M	M	NA	Agree	0.04	7.23	0.00	42.79	24.31	70.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865325	0.080774	0.042176	0.011725	91.52	3.03	5.24	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586709	M	M	NA	Agree	0.01	7.26	0.00	34.05	18.26	64.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85563	0.072601	0.039564	0.032205	88.53	5.22	6.01	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586710	M	M	NA	Agree	0.01	7.96	0.00	52.51	27.88	64.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855606	0.091163	0.044741	0.008491	91.33	2.67	5.75	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586711	M	M	NA	Agree	0.00	5.38	0.00	40.04	18.04	50.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881254	0.066176	0.042207	0.010363	91.17	3.02	5.53	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586712	M	M	NA	Agree	0.00	4.71	0.00	37.36	16.38	54.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895301	0.056034	0.039185	0.009481	89.27	2.99	7.50	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586713	M	M	NA	Agree	0.00	8.93	0.00	45.20	23.96	65.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849909	0.093997	0.040468	0.015626	89.29	3.15	7.36	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586714	M	M	NA	Agree	0.00	4.76	0.00	26.66	19.38	57.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890899	0.058833	0.041058	0.00921	90.35	2.79	6.64	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586715	M	M	NA	Agree	0.01	2.13	0.00	12.80	5.95	33.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877055	0.043485	0.035004	0.044457	84.24	6.04	9.50	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does