Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8586263	M	M	NA	Agree	0.02	4.28	0.00	29.00	9.79	68.72	NONE	POLYA	0.922267	0.031614	0.031756	0.014363	93.85	3.21	2.60	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586264	M	M	NA	Agree	0.04	3.81	0.00	25.42	9.57	36.50	NONE	POLYA	0.844041	0.118074	0.033512	0.004373	89.13	3.59	5.80	179	LP2 (2026-09-30)	PASS	NonPolyA_perM 179 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586266	M	M	NA	Agree	0.00	6.94	0.00	37.84	11.73	63.09	NONE	POLYA	0.910361	0.050075	0.03752	0.002044	96.38	1.60	1.86	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586267	M	M	NA	Agree	0.00	5.61	0.00	28.78	13.27	48.68	NONE	POLYA	0.944224	0.024539	0.029005	0.002231	96.55	1.46	1.81	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586269	M	M	NA	Agree	0.00	7.82	0.00	35.26	10.12	65.46	NONE	POLYA	0.932099	0.032224	0.032593	0.003084	95.64	1.96	2.24	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586270	M	M	NA	Agree	0.01	6.73	0.00	30.49	10.95	61.50	NONE	POLYA	0.924272	0.041355	0.032582	0.001792	95.03	1.82	2.73	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586272	M	M	NA	Agree	0.03	4.26	0.00	22.83	8.12	49.66	NONE	POLYA	0.922195	0.04329	0.031333	0.003182	92.69	2.73	3.62	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8586273	M	M	NA	Agree	0.04	3.49	0.00	20.43	7.17	46.48	NONE	POLYA	0.919032	0.048002	0.030435	0.00253	90.19	3.51	5.05	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does