Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8563008	M	M	NA	Agree	0.01	8.07	0.00	39.01	16.64	68.60	NONE	POLYA	0.827248	0.121177	0.044179	0.007397	87.02	1.98	10.79	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563009	M	M	NA	Agree	0.02	7.05	0.00	36.99	16.03	61.13	NONE	POLYA	0.841578	0.112852	0.043606	0.001964	87.79	1.45	10.56	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563010	M	M	NA	Agree	0.00	6.88	0.00	43.88	18.17	63.04	NONE	POLYA	0.838824	0.116929	0.042293	0.001953	88.45	1.54	9.80	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563011	M	M	NA	Agree	0.00	8.12	0.00	37.13	18.31	58.13	NONE	POLYA	0.831513	0.121926	0.044678	0.001883	89.74	1.43	8.62	144	LP2 (2026-09-30)	PASS	NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563012	M	M	NA	Agree	0.01	7.84	0.00	32.64	16.88	63.43	NONE	POLYA	0.832897	0.118421	0.046611	0.002071	89.11	1.63	9.06	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563013	M	M	NA	Agree	0.00	7.07	0.00	40.18	20.21	57.35	NONE	POLYA	0.820075	0.131133	0.045338	0.003454	87.36	1.58	10.83	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563014	M	M	NA	Agree	0.05	5.98	0.00	30.57	15.90	44.66	NONE	POLYA	0.809725	0.137875	0.040743	0.011657	87.49	2.32	9.99	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563015	M	M	NA	Agree	0.00	6.38	0.00	40.27	17.49	62.49	NONE	POLYA	0.821661	0.129748	0.044737	0.003855	87.84	1.64	10.29	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563016	M	M	NA	Agree	0.00	7.70	0.00	40.04	15.95	73.90	NONE	POLYA	0.828079	0.124652	0.044327	0.002943	87.78	1.55	10.48	136	LP2 (2026-09-30)	PASS	NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563017	M	M	NA	Agree	0.00	7.44	0.00	37.25	15.93	71.89	NONE	POLYA	0.83443	0.118576	0.043616	0.003378	82.45	1.57	15.77	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563018	M	M	NA	Agree	0.02	5.47	0.00	47.56	14.92	71.86	NONE	POLYA	0.85078	0.102744	0.042552	0.003924	87.96	1.76	10.07	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563019	M	M	NA	Agree	0.00	7.51	0.00	38.10	18.66	64.53	NONE	POLYA	0.839479	0.114241	0.044534	0.001746	88.60	1.43	9.77	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563020	M	M	NA	Agree	0.01	6.19	0.00	34.63	15.54	66.03	NONE	POLYA	0.849297	0.104247	0.043595	0.002861	88.54	1.59	9.68	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563021	M	M	NA	Agree	0.00	6.08	0.00	38.26	18.08	56.02	NONE	POLYA	0.839937	0.113487	0.044765	0.001811	88.18	1.50	10.09	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563022	M	M	NA	Agree	0.01	4.12	0.00	28.01	15.13	45.42	NONE	POLYA	0.857419	0.101165	0.040105	0.00131	89.17	1.33	9.32	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563023	M	M	NA	Agree	0.01	7.84	0.00	40.04	16.52	64.19	NONE	POLYA	0.835806	0.118676	0.043759	0.001758	88.44	1.40	9.94	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563024	M	M	NA	Agree	0.01	7.75	0.00	44.68	19.06	60.92	NONE	POLYA	0.8235	0.130429	0.044586	0.001486	88.63	1.39	9.77	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563025	M	M	NA	Agree	0.03	8.28	0.00	40.89	17.15	71.13	NONE	POLYA	0.835669	0.116069	0.044575	0.003686	87.15	1.63	10.98	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563026	M	M	NA	Agree	0.00	5.30	0.00	44.73	15.69	66.72	NONE	POLYA	0.844537	0.110512	0.042308	0.002643	89.09	1.59	9.11	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563027	M	M	NA	Agree	0.00	6.73	0.00	39.03	17.72	71.42	NONE	POLYA	0.835829	0.117692	0.044	0.002479	87.63	1.54	10.59	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563028	M	M	NA	Agree	0.00	8.34	0.00	39.09	17.88	61.80	NONE	POLYA	0.832088	0.121072	0.044859	0.001981	89.48	1.42	8.92	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563029	M	M	NA	Agree	0.00	7.10	0.00	41.00	16.71	67.48	NONE	POLYA	0.843513	0.106841	0.044315	0.005331	89.11	1.85	8.82	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563030	M	M	NA	Agree	0.00	5.21	0.00	32.07	18.86	47.22	NONE	POLYA	0.841375	0.116223	0.040149	0.002253	89.49	1.39	8.93	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563031	M	M	NA	Agree	0.01	5.65	0.00	32.28	11.57	65.46	NONE	POLYA	0.891912	0.06132	0.042908	0.003859	88.33	1.82	9.69	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563032	M	M	NA	Agree	0.04	6.90	0.00	44.70	16.34	70.67	NONE	POLYA	0.844744	0.106512	0.043016	0.005729	88.91	1.79	9.13	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563033	M	M	NA	Agree	0.00	5.96	0.00	46.79	18.70	67.43	NONE	POLYA	0.832576	0.121247	0.044432	0.001745	88.86	1.50	9.42	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563034	M	M	NA	Agree	0.00	5.34	0.00	44.88	14.18	58.99	NONE	POLYA	0.784525	0.10049	0.041615	0.07337	82.56	8.07	9.15	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563035	M	M	NA	Agree	0.01	2.99	0.00	36.01	16.36	54.29	NONE	POLYA	0.825702	0.126617	0.045088	0.002593	87.51	1.55	10.75	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563036	M	M	NA	Agree	0.01	8.32	0.00	36.94	17.47	63.01	NONE	POLYA	0.836587	0.116927	0.044427	0.002059	88.73	1.46	9.62	136	LP2 (2026-09-30)	PASS	NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563037	M	M	NA	Agree	0.01	8.06	0.00	36.99	19.08	59.76	NONE	POLYA	0.832437	0.121495	0.044539	0.001529	88.53	1.39	9.87	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563038	M	M	NA	Agree	0.00	5.58	0.00	33.90	14.81	43.63	NONE	POLYA	0.844991	0.110029	0.042164	0.002816	87.78	1.48	10.54	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8563039	M	M	NA	Agree	0.02	7.00	0.00	41.96	17.75	57.36	NONE	POLYA	0.828777	0.12408	0.045412	0.001731	87.75	1.43	10.62	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does