Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8551864	F	F	NA	Agree	188.33	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.857153	0.096102	0.042866	0.00388	85.62	1.50	12.72	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551865	F	F	NA	Agree	169.77	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846878	0.099859	0.050524	0.002739	89.29	1.46	9.08	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551866	M	M	NA	Agree	0.70	11.37	0.00	56.13	23.89	100.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850455	0.098289	0.045016	0.00624	91.45	1.90	6.45	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551867	M	M	NA	Agree	0.05	10.86	0.00	50.62	29.93	97.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848884	0.097729	0.050057	0.00333	89.06	1.54	9.23	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551868	F	F	NA	Agree	148.64	0.05	0.00	0.30	0.07	0.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858478	0.091623	0.044166	0.005733	89.03	1.81	8.98	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551869	M	M	NA	Agree	0.35	13.04	0.00	63.08	27.02	110.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849039	0.099235	0.045933	0.005793	90.46	1.84	7.51	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551870	M	M	NA	Agree	0.02	10.47	0.00	51.25	27.36	96.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855629	0.093315	0.048735	0.002321	90.13	1.48	8.21	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551871	F	F	NA	Agree	176.15	0.01	0.00	0.02	0.02	0.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849229	0.096807	0.049086	0.004878	88.65	1.64	9.54	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551872	M	M	NA	Agree	0.73	10.39	0.00	56.67	37.90	107.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852113	0.09514	0.048485	0.004262	87.23	1.55	11.04	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551873	M	M	NA	Agree	0.05	11.99	0.00	52.24	25.78	96.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844966	0.098493	0.044377	0.012164	85.95	2.41	11.45	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551874	F	F	NA	Agree	113.32	0.02	0.00	0.12	0.04	0.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861395	0.08761	0.043473	0.007521	89.18	1.99	8.65	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551875	F	F	NA	Agree	100.53	0.03	0.00	0.12	0.08	0.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854763	0.089544	0.044159	0.011533	85.27	2.24	12.33	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551876	M	M	NA	Agree	0.26	6.16	0.00	28.72	19.18	66.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869298	0.078279	0.042256	0.010167	84.16	2.12	13.58	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551877	M	M	NA	Agree	0.03	10.10	0.00	47.32	24.53	102.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848736	0.095954	0.044732	0.010578	89.48	2.36	7.97	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551878	F	F	NA	Agree	131.83	0.03	0.00	0.28	0.17	0.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852397	0.094859	0.04422	0.008524	91.81	2.19	5.81	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551879	F	F	NA	Agree	191.64	0.05	0.00	0.06	0.04	0.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837901	0.102515	0.053087	0.006497	92.36	1.92	5.54	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551880	M	M	NA	Agree	0.00	8.58	0.00	51.12	26.59	87.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867096	0.088758	0.043428	0.000718	85.37	1.25	13.20	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551881	M	M	NA	Agree	0.02	12.66	0.00	57.78	32.59	111.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854951	0.094998	0.049572	0.000479	90.20	1.36	8.27	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551882	M	M	NA	Agree	0.01	12.09	0.00	59.37	31.15	101.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855711	0.095164	0.048598	0.000527	88.43	1.31	10.09	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551883	M	M	NA	Agree	0.02	12.04	0.00	58.91	26.43	110.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.857711	0.093377	0.047692	0.00122	89.75	1.45	8.61	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551884	M	M	NA	Agree	0.02	12.75	0.00	56.98	29.01	101.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858388	0.093527	0.047558	0.000528	90.59	1.31	7.94	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551885	M	M	NA	Agree	0.01	11.09	0.00	55.37	26.21	102.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858029	0.093277	0.047629	0.001064	93.04	1.38	5.41	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551886	M	M	NA	Agree	0.01	10.77	0.00	60.75	29.10	103.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862344	0.091332	0.045629	0.000695	90.45	1.36	7.99	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551887	M	M	NA	Agree	0.02	10.26	0.00	54.08	25.05	89.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863629	0.090333	0.045289	0.000749	90.59	1.37	7.85	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551888	F	M	NA	Conflict	0.01	10.46	0.00	43.45	24.32	109.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868709	0.081181	0.049368	0.000742	92.25	1.51	6.03	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551889	F	F	NA	Agree	121.33	0.01	0.00	0.03	0.08	0.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875153	0.082626	0.041452	0.000769	83.69	1.24	14.89	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551890	F	F	NA	Agree	152.86	0.00	0.00	0.01	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856417	0.094792	0.048258	0.000533	87.58	1.27	10.99	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551891	F	F	NA	Agree	152.73	0.04	0.00	0.00	0.00	0.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864315	0.088936	0.046066	0.000682	92.68	1.38	5.74	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551892	F	F	NA	Agree	141.36	0.00	0.00	0.04	0.00	0.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863752	0.088441	0.045798	0.00201	90.05	1.53	8.24	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551893	F	F	NA	Agree	151.88	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858221	0.092488	0.048789	0.000501	89.60	1.29	8.93	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551894	F	F	NA	Agree	164.48	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854279	0.094094	0.050695	0.000932	87.48	1.34	11.02	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8551895	F	F	NA	Agree	192.58	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855446	0.095134	0.048558	0.000862	93.00	1.34	5.47	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does