Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8437133	not_specified	M	NA	Conflict	0.01	5.41	0.00	27.59	12.92	79.61	NONE	POLYA	0.929336	0.021958	0.043298	0.005408	89.36	1.93	8.58	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437134	not_specified	M	NA	Conflict	0.04	5.23	0.00	27.44	11.57	80.12	NONE	POLYA	0.929777	0.021843	0.043298	0.005082	89.36	1.85	8.65	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437135	not_specified	M	NA	Conflict	0.00	6.50	0.00	31.27	11.17	86.20	NONE	POLYA	0.930772	0.02269	0.042908	0.00363	91.96	1.79	6.11	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437136	not_specified	M	NA	Conflict	0.00	5.19	0.00	25.66	12.09	83.64	NONE	POLYA	0.929726	0.022217	0.042745	0.005312	90.47	1.98	7.42	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437137	not_specified	M	NA	Conflict	0.02	5.86	0.00	29.85	12.11	72.74	NONE	POLYA	0.923892	0.023255	0.044708	0.008145	89.33	2.26	8.29	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437138	not_specified	M	NA	Conflict	0.04	3.34	0.00	14.99	9.57	68.15	NONE	POLYA	0.921255	0.022822	0.048019	0.007904	89.89	2.35	7.62	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437139	not_specified	M	NA	Conflict	0.03	5.22	0.00	24.97	11.51	90.83	NONE	POLYA	0.919252	0.024059	0.046512	0.010177	90.25	2.46	7.19	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437140	not_specified	M	NA	Conflict	0.03	3.16	0.00	17.94	11.12	57.61	NONE	POLYA	0.929957	0.021039	0.0418	0.007204	87.94	2.10	9.83	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437141	not_specified	M	NA	Conflict	0.00	5.05	0.00	25.30	10.29	74.44	NONE	POLYA	0.928006	0.021294	0.045839	0.004862	90.77	1.97	7.14	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437142	not_specified	M	NA	Conflict	0.07	5.06	0.00	33.36	12.32	88.28	NONE	POLYA	0.924909	0.023521	0.046918	0.004651	92.47	1.94	5.47	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437143	not_specified	M	NA	Conflict	0.01	4.50	0.00	25.12	11.66	79.21	NONE	POLYA	0.929092	0.020149	0.045926	0.004833	90.77	1.98	7.13	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437144	not_specified	M	NA	Conflict	0.04	7.04	0.00	30.20	12.48	87.53	NONE	POLYA	0.923908	0.025142	0.047148	0.003802	91.70	1.86	6.32	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437145	not_specified	M	NA	Conflict	0.02	3.44	0.00	17.30	10.02	68.63	NONE	POLYA	0.929441	0.020629	0.04234	0.00759	91.54	2.25	6.08	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437146	not_specified	M	NA	Conflict	0.05	3.19	0.00	15.53	9.00	64.12	NONE	POLYA	0.919158	0.027916	0.043867	0.009059	90.50	2.22	7.17	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437147	not_specified	M	NA	Conflict	0.00	4.08	0.00	21.78	10.92	65.66	NONE	POLYA	0.928381	0.021983	0.044004	0.005631	91.73	2.02	6.13	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437148	not_specified	M	NA	Conflict	0.02	6.51	0.00	30.95	9.53	49.57	NONE	POLYA	0.899665	0.039257	0.03909	0.021988	87.08	4.30	8.48	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437149	not_specified	M	NA	Conflict	0.01	5.46	0.00	26.34	12.32	68.44	NONE	POLYA	0.929516	0.022569	0.044231	0.003684	89.64	1.87	8.37	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8437150	not_specified	M	NA	Conflict	0.04	5.27	0.00	28.15	11.65	73.38	NONE	POLYA	0.929515	0.022299	0.043597	0.004589	90.23	1.86	7.80	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does