Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8421922	not_specified	M	NA	Conflict	0.04	6.89	0.00	35.75	16.61	72.71	NONE	POLYA	0.90094	0.05229	0.044978	0.001792	88.12	1.44	10.31	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421923	not_specified	F	NA	Conflict	168.55	0.00	0.00	0.00	0.10	0.00	NONE	POLYA	0.890032	0.06081	0.04673	0.002428	88.31	1.50	10.04	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421924	not_specified	M	NA	Conflict	0.00	6.39	0.00	40.54	15.20	71.89	NONE	POLYA	0.896382	0.055912	0.045125	0.002581	88.09	1.51	10.26	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421925	not_specified	F	NA	Conflict	127.99	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.904491	0.048514	0.044656	0.002339	87.95	1.52	10.39	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421926	not_specified	M	NA	Conflict	0.00	7.57	0.00	35.88	17.85	60.20	NONE	POLYA	0.897833	0.055374	0.044923	0.00187	85.43	1.44	13.00	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421927	not_specified	M	NA	Conflict	0.00	5.58	0.00	35.11	13.51	74.23	NONE	POLYA	0.911833	0.042951	0.043173	0.002044	83.88	1.48	14.51	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421928	not_specified	F	NA	Conflict	134.06	0.00	0.00	0.06	0.00	0.00	NONE	POLYA	0.899675	0.054209	0.044405	0.00171	87.14	1.45	11.27	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421929	not_specified	F	NA	Conflict	135.69	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.900683	0.052561	0.04494	0.001816	86.96	1.46	11.45	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421930	not_specified	M	NA	Conflict	0.00	15.95	0.00	64.54	23.46	109.30	NONE	POLYA	0.876214	0.060213	0.055287	0.008287	87.40	2.14	10.31	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421931	not_specified	M	NA	Conflict	0.04	18.60	0.00	52.44	24.06	88.51	NONE	POLYA	0.871533	0.067395	0.053185	0.007887	89.12	1.99	8.74	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421932	not_specified	M	NA	Conflict	0.00	12.87	0.00	57.95	20.77	101.46	NONE	POLYA	0.882121	0.053869	0.053064	0.010946	88.70	2.35	8.80	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421933	not_specified	M	NA	Conflict	0.00	15.20	0.00	56.05	28.91	106.44	NONE	POLYA	0.861765	0.069674	0.057166	0.011395	90.66	2.39	6.80	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421934	not_specified	F	NA	Conflict	117.00	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.888321	0.053506	0.045	0.013173	93.11	2.49	4.24	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421935	not_specified	F	NA	Conflict	149.81	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.876625	0.062673	0.048406	0.012295	93.64	2.36	3.86	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421936	not_specified	M	NA	Conflict	0.00	7.04	0.00	40.81	23.10	72.35	NONE	POLYA	0.869269	0.068614	0.048621	0.013497	93.19	2.46	4.19	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421937	not_specified	M	NA	Conflict	0.10	11.43	0.00	47.09	18.40	80.93	NONE	POLYA	0.890317	0.04986	0.047967	0.011856	92.43	2.39	5.04	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421938	not_specified	M	NA	Conflict	0.09	5.17	0.00	36.48	12.89	69.40	NONE	POLYA	0.914548	0.038381	0.044432	0.002639	88.60	1.50	9.77	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421939	not_specified	F	NA	Conflict	120.49	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.89972	0.052022	0.045779	0.002479	84.07	1.52	14.27	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421940	not_specified	F	NA	Conflict	102.22	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.90557	0.046467	0.044746	0.003217	87.08	1.60	11.18	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421941	not_specified	F	NA	Conflict	93.47	0.00	0.00	0.37	0.00	0.00	NONE	POLYA	0.913473	0.039737	0.044346	0.002443	82.16	1.47	16.24	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421942	not_specified	F	NA	Conflict	132.41	0.00	0.00	0.00	0.00	0.10	NONE	POLYA	0.898818	0.0535	0.045607	0.002075	88.55	1.58	9.74	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421943	not_specified	M	NA	Conflict	0.10	5.88	0.00	36.97	11.75	59.96	NONE	POLYA	0.901857	0.051384	0.044415	0.002344	89.14	1.65	9.07	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421944	not_specified	F	NA	Conflict	202.65	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.888405	0.064779	0.044957	0.001859	87.96	1.44	10.46	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421945	not_specified	F	NA	Conflict	158.99	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.898751	0.053767	0.044917	0.002565	88.67	1.57	9.63	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421946	not_specified	F	NA	Conflict	211.64	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.869702	0.073603	0.047343	0.009352	93.13	2.32	4.38	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421947	not_specified	F	NA	Conflict	324.07	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.852072	0.088835	0.052025	0.007068	91.26	2.02	6.56	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421948	not_specified	M	NA	Conflict	0.03	6.52	0.00	34.47	12.02	62.54	NONE	POLYA	0.899581	0.039236	0.044432	0.016751	92.67	2.78	4.41	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421949	not_specified	M	NA	Conflict	0.08	7.42	0.00	37.16	13.67	61.33	NONE	POLYA	0.88537	0.057758	0.046529	0.010344	93.29	2.21	4.36	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421950	not_specified	F	NA	Conflict	132.80	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.88838	0.052713	0.051505	0.007402	90.82	2.04	6.97	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421951	not_specified	F	NA	Conflict	157.07	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.890331	0.049701	0.051941	0.008028	91.36	2.18	6.30	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421952	not_specified	M	NA	Conflict	0.13	11.53	0.00	48.09	18.81	74.09	NONE	POLYA	0.884472	0.057669	0.05037	0.007489	94.35	2.01	3.48	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8421953	not_specified	M	NA	Conflict	0.09	9.94	0.00	42.66	15.87	83.00	NONE	POLYA	0.879794	0.061289	0.049338	0.009579	92.48	2.06	5.31	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does