Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8374118	not_specified	M	NA	Conflict	0.09	4.31	0.00	29.83	18.77	37.95	NONE	POLYA	0.862104	0.096991	0.037154	0.00375	93.96	2.09	3.62	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374119	not_specified	M	NA	Conflict	0.08	3.81	0.00	25.92	19.71	47.42	NONE	POLYA	0.860089	0.096814	0.039272	0.003825	94.05	2.28	3.27	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374120	not_specified	M	NA	Conflict	0.01	3.88	0.00	25.49	17.41	46.48	NONE	POLYA	0.877814	0.0798	0.038388	0.003998	93.52	2.31	3.76	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374121	not_specified	M	NA	Conflict	0.04	4.27	0.00	25.10	16.38	49.16	NONE	POLYA	0.86328	0.095609	0.037184	0.003927	93.69	2.37	3.51	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374122	not_specified	M	NA	Conflict	0.07	3.30	0.00	27.07	16.83	43.95	NONE	POLYA	0.849477	0.10671	0.03916	0.004653	93.57	2.31	3.71	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374123	not_specified	M	NA	Conflict	0.06	3.12	0.00	26.69	16.95	41.03	NONE	POLYA	0.865106	0.092527	0.037579	0.004788	93.66	2.35	3.58	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374124	not_specified	M	NA	Conflict	0.04	2.55	0.00	23.67	14.29	44.80	NONE	POLYA	0.854173	0.103148	0.037337	0.005343	93.43	2.32	3.87	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374125	not_specified	M	NA	Conflict	0.04	3.52	0.00	24.29	14.50	41.27	NONE	POLYA	0.861923	0.096986	0.036507	0.004583	93.61	2.40	3.53	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374126	not_specified	M	NA	Conflict	0.07	3.39	0.00	22.39	17.50	38.53	NONE	POLYA	0.870372	0.087835	0.037905	0.003889	93.89	2.28	3.43	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374127	not_specified	M	NA	Conflict	0.01	4.36	0.00	24.37	14.48	42.11	NONE	POLYA	0.886194	0.075008	0.033219	0.00558	93.09	2.58	3.93	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374128	not_specified	M	NA	Conflict	0.04	5.40	0.00	24.70	18.71	51.45	NONE	POLYA	0.88549	0.072726	0.037931	0.003852	93.52	2.30	3.80	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8374129	not_specified	M	NA	Conflict	0.02	3.64	0.00	26.77	15.83	35.37	NONE	POLYA	0.864823	0.093618	0.038298	0.003262	93.20	2.23	4.17	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does