Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8367693	M	M	NA	Agree	0.00	2.02	0.00	21.98	5.63	30.97	NONE	POLYA	0.908687	0.067636	0.023567	0.00011	95.88	2.29	1.18	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367694	M	M	NA	Agree	0.00	2.26	0.00	19.70	5.79	29.72	NONE	POLYA	0.90179	0.074033	0.02407	0.000107	96.17	1.97	1.23	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367695	M	M	NA	Agree	0.00	2.61	0.00	21.31	6.03	32.22	NONE	POLYA	0.906929	0.068952	0.024028	0.000091	96.08	2.01	1.28	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367696	M	M	NA	Agree	0.01	1.82	0.00	21.34	6.23	35.29	NONE	POLYA	0.905246	0.070792	0.023796	0.000166	96.04	2.18	1.17	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367697	M	M	NA	Agree	0.00	2.18	0.00	23.13	5.07	34.46	NONE	POLYA	0.907355	0.067308	0.025218	0.000119	95.62	2.25	1.31	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367698	M	M	NA	Agree	0.00	2.34	0.00	22.86	5.72	36.53	NONE	POLYA	0.908677	0.067241	0.023987	0.000095	95.71	2.30	1.30	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367699	M	M	NA	Agree	0.00	2.15	0.00	19.71	5.76	34.12	NONE	POLYA	0.905689	0.070931	0.023268	0.000112	95.71	2.26	1.27	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367700	M	M	NA	Agree	0.00	2.84	0.00	21.69	5.75	31.62	NONE	POLYA	0.905722	0.070085	0.024129	0.000064	95.94	2.05	1.34	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367701	M	M	NA	Agree	0.00	1.97	0.00	19.80	5.96	30.31	NONE	POLYA	0.899553	0.074321	0.026021	0.000105	96.04	2.11	1.29	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367702	M	M	NA	Agree	0.00	2.00	0.00	21.47	6.69	32.74	NONE	POLYA	0.911787	0.064366	0.023742	0.000106	96.07	2.04	1.24	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367703	M	M	NA	Agree	0.00	2.01	0.00	22.67	6.88	36.09	NONE	POLYA	0.916634	0.058375	0.024885	0.000106	96.19	1.98	1.23	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367704	M	M	NA	Agree	0.00	2.38	0.00	20.66	6.10	31.09	NONE	POLYA	0.905416	0.070431	0.024031	0.000121	96.16	2.08	1.21	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367705	M	M	NA	Agree	0.00	1.97	0.00	19.66	6.88	29.68	NONE	POLYA	0.916819	0.058397	0.024618	0.000166	96.01	2.13	1.31	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367706	M	M	NA	Agree	0.00	2.78	0.00	20.24	6.42	34.13	NONE	POLYA	0.908555	0.067477	0.023851	0.000117	95.95	2.20	1.24	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367707	M	M	NA	Agree	0.00	2.38	0.00	24.70	7.77	39.24	NONE	POLYA	0.902198	0.071677	0.026028	0.000097	96.06	1.99	1.40	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8367708	M	M	NA	Agree	0.00	2.16	0.00	21.66	5.50	31.74	NONE	POLYA	0.913541	0.063165	0.023197	0.000097	95.84	2.15	1.37	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does