Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8319641	M	M	NA	Agree	0.00	4.13	0.00	48.84	5.69	50.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867008	0.030134	0.027051	0.075807	65.99	7.19	26.48	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8319642	M	M	NA	Agree	0.02	6.50	0.00	53.23	6.60	57.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869774	0.042243	0.032528	0.055455	75.03	6.30	18.40	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8319643	M	M	NA	Agree	0.00	4.90	0.00	51.95	5.84	44.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.824974	0.036903	0.029439	0.108685	60.38	9.08	30.23	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8319644	M	M	NA	Agree	0.00	6.54	0.00	35.83	7.98	53.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877618	0.037692	0.03503	0.04966	73.91	5.66	20.16	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8319645	M	M	NA	Agree	0.01	5.82	0.00	33.29	8.46	50.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.897603	0.04106	0.031943	0.029394	72.90	3.80	23.01	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8319646	M	M	NA	Agree	0.00	4.44	0.00	21.04	4.65	55.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.816858	0.029356	0.024433	0.129353	53.22	9.40	36.98	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8319647	M	M	NA	Agree	0.01	6.35	0.00	32.88	7.76	55.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834632	0.044279	0.031635	0.089454	62.37	7.73	29.58	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8319648	M	M	NA	Agree	0.00	5.69	0.00	40.23	11.52	47.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.790369	0.046815	0.029725	0.133091	60.51	11.04	28.18	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does