Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8259910	M	M	NA	Agree	0.00	1.16	0.00	15.25	3.04	25.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.95265	0.018479	0.017859	0.011012	88.96	3.16	7.62	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259911	M	M	NA	Agree	0.00	1.23	0.00	19.15	3.35	31.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.945143	0.019238	0.018399	0.01722	90.91	3.79	5.07	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259912	M	M	NA	Agree	0.00	1.89	0.00	14.16	3.38	36.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.958248	0.014372	0.020706	0.006675	89.59	2.87	7.33	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259913	M	M	NA	Agree	0.00	1.69	0.00	16.55	3.60	38.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.958234	0.014307	0.021122	0.006337	90.38	2.88	6.54	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259914	M	M	NA	Agree	0.00	1.32	0.00	20.44	3.23	34.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.958453	0.014571	0.020889	0.006087	89.30	2.88	7.58	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259915	M	M	NA	Agree	0.00	1.69	0.00	23.80	3.31	37.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.956154	0.015411	0.0202	0.008235	91.31	3.05	5.37	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259916	M	M	NA	Agree	0.00	1.76	0.00	28.96	3.91	38.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.959424	0.016271	0.019716	0.004589	93.68	2.68	3.33	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259917	M	M	NA	Agree	0.00	1.13	0.00	10.49	2.12	34.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.960031	0.015118	0.017824	0.007026	91.55	2.94	5.30	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259918	M	M	NA	Agree	0.00	0.94	0.00	8.25	2.08	32.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.958994	0.014547	0.017105	0.009354	93.18	3.28	3.30	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259919	M	M	NA	Agree	0.00	0.84	0.00	13.05	2.50	32.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.959231	0.014678	0.018051	0.00804	91.99	3.23	4.50	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259920	M	M	NA	Agree	0.00	0.73	0.00	8.71	3.08	23.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.947886	0.023652	0.0174	0.011063	91.12	3.29	5.32	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259921	M	M	NA	Agree	0.00	1.34	0.00	22.09	2.37	27.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.961065	0.012376	0.017897	0.008661	90.69	2.98	6.07	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259922	M	M	NA	Agree	0.00	1.46	0.00	11.50	3.18	44.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930552	0.015344	0.021743	0.032361	89.75	5.40	4.70	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259923	M	M	NA	Agree	0.01	1.78	0.00	16.41	3.55	40.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.957846	0.014428	0.021122	0.006604	91.57	2.94	5.29	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8259924	M	M	NA	Agree	0.00	1.67	0.00	24.01	4.01	39.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.958154	0.014325	0.02137	0.006151	91.86	2.83	5.09	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does