Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8255521	M	M	NA	Agree	0.02	3.14	0.00	41.87	12.58	54.29	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.874711	0.088839	0.033344	0.003106	92.93	2.01	4.78	167	LP2 (2026-09-30)	PASS	NonPolyA_perM 167 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255522	M	M	NA	Agree	0.02	4.54	0.00	42.80	12.57	56.01	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.877742	0.087976	0.032883	0.001398	93.31	1.83	4.58	166	LP2 (2026-09-30)	PASS	NonPolyA_perM 166 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255523	M	M	NA	Agree	0.06	3.22	0.00	45.60	13.87	58.92	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.875651	0.089176	0.03298	0.002193	93.15	1.94	4.63	154	LP2 (2026-09-30)	PASS	NonPolyA_perM 154 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255524	M	M	NA	Agree	0.03	1.88	0.00	31.20	7.94	43.13	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.907951	0.066258	0.02469	0.001101	94.51	2.10	3.18	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255525	M	M	NA	Agree	0.05	2.38	0.00	29.66	8.32	43.98	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.908259	0.065558	0.024669	0.001514	94.45	2.18	3.15	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255526	M	M	NA	Agree	0.02	2.65	0.00	34.42	8.28	44.30	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.90424	0.068572	0.025419	0.001769	94.45	2.20	3.12	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255527	M	M	NA	Agree	0.06	2.03	0.00	17.10	8.35	57.44	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.892033	0.074492	0.031481	0.001995	93.62	2.29	3.77	164	LP2 (2026-09-30)	PASS	NonPolyA_perM 164 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255528	M	M	NA	Agree	0.02	2.57	0.00	17.58	8.20	59.53	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.891788	0.07495	0.031301	0.001961	93.58	2.28	3.82	158	LP2 (2026-09-30)	PASS	NonPolyA_perM 158 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8255529	M	M	NA	Agree	0.03	2.16	0.00	19.39	8.23	63.44	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.892791	0.072604	0.030628	0.003976	93.51	2.48	3.70	155	LP2 (2026-09-30)	PASS	NonPolyA_perM 155 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does